Bioaccessible and dialysable polyphenols in selected apple varieties following in vitro digestion vs. their native patterns |
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Authors: | Jaouad Bouayed Hannah DeußerLucien Hoffmann Torsten Bohn |
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Affiliation: | Centre de Recherche Public - Gabriel Lippmann, Environment and Agro-Biotechnologies Department, 41, rue du Brill, L-4422 Belvaux, Luxembourg |
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Abstract: | To assess bioaccessible and dialysable apple polyphenols available for potential uptake by intestinal epithelial cells, an in vitro gastrointestinal (GI) digestion method was developed and main polyphenols investigated by UPLC. Polyphenolic profiles in the gastric medium were similar to those natively occurring in apples; however, bioaccessible polyphenols were at lower concentrations than those in the apples. The polyphenolic profile was altered during intestinal digestion, with a considerable decrease of total polyphenols. Flavan-3-ols were completely unstable in the intestinal medium, owing to their pH sensitivity. In addition, 41-77% of bioaccessible chlorogenic acid, the major abundant hydroxycinnamic acid in apples, was degraded during intestinal digestion, with partial isomerisation to cryptochlorogenic acid and neochlorogenic acid. All polyphenols found in the intestinal medium were dialysable, but were present at lower concentrations, suggesting that dialysable polyphenols can potentially be taken up by the enterocytes. These results highlight that GI digestion may substantially affect native apple-derived polyphenolic patterns and concentrations. |
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Keywords: | Apples Polyphenols Gastrointestinal digestion Extraction Bioaccessibility Dialysability |
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