Identification of phenolic constituents of Cytisus multiflorus |
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Authors: | Olívia R Pereira Artur MS SilvaMaria RM Domingues Susana M Cardoso |
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Affiliation: | a CERNAS - Escola Superior Agrária, Instituto Politécnico de Coimbra, Bencanta, 3040-316 Coimbra, Portugal b Departamento de Tecnologias de Diagnóstico e Terapêutica, Escola Superior de Saúde, Instituto Politécnico de Bragança, Av. D. Afonso V, 5300-121 Bragança, Portugal c Departamento de Química & QOPNA, Universidade de Aveiro, 3810-193 Aveiro, Portugal d CIMO - Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1038, 5301-854 Bragança, Portugal |
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Abstract: | The phenolic composition of the ethanolic extract obtained from the flowers of the medicinal plant Cytisus multiflorus has been elucidated by high performance liquid chromatography, electrospray mass spectrometry and nuclear magnetic resonance analysis. The extract was mainly composed of flavones, including the common chrysin, orientin, luteolin-5-O-glucoside, luteolin-7-O-glucoside, apigenin and apigenin-7-O-glucoside, which appeared as minor components. The major flavone in the extract was chrysin-7-O-β-d-glucopyranoside, and it also contained moderate amounts of a dihydroxyflavone isomer of chrysin, as well as of 2″-O-pentosyl-6-C-hexosyl-luteolin, 2″-O-pentosyl-8-C-hexosyl-luteolin and 6″-O-(3-hydroxy-3-methylglutaroyl)-2″-O-pentosyl-C-hexosyl-apigenin, which are not commonly found in the Fabaceae family. Other novel phenolic compounds found in the ethanolic extract of C. multiflorus comprised the flavones 2″-O-pentosyl-6-C-hexosyl-apigenin, 2″-O-pentosyl-8-C-hexosyl-apigenin and 6″-O-(3-hydroxy-3-methylglutaroyl)-2″-O-pentosyl-C-hexosyl-luteolin. The assessment of the biological activities of the main compounds of this extract are now keen, in order to determine their relevance in the beneficial properties of the plant. |
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Keywords: | CID collision-induced dissociation DAD diode array ESI-MS electrospray ionisation-mass spectrometry MSn Tandem mass spectrometry GAE gallic acid equivalent HPLC high-performance liquid chromatography NMR nuclear magnetic resonance |
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