| Screening, identification and desilication of a silicate bacterium |
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| 作者姓名： | 周洪波 曾晓希 刘飞飞 邱冠周 胡岳华 |
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| 作者单位： | School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China |
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| 基金项目： | 中国科学院资助项目;国家重点基础研究发展计划(973计划) |
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| 摘 要： | The strain Lv（1- z） isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite concentration on the desilicating ability of the strain Lv（1- z） were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv（1- z） is similar to Bacillus rnucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv（1- z） and the B. rnucilaginosus YNUCC0001（AY571332） is more than 99%. Based on the above results, the strain Lv（1- z） is identified as B. rnucilaginosus. The optimum conditions for the strain L（1- z） to remove silicon from illite are as follows., temperature is 30℃ ;initial pH value is 7.5; medium volume in 200 mL bottle is 60 mL; shaking speed of rotary shaker is 220 r/m ; illite concentration is 1%.
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| 关 键 词： | 筛选 鉴定 硅迁移 杆状菌 脱硅作用 |
| 文章编号： | 1005-9784（2006）04-0337-05 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-02-20 |
Screening, identification and desilication of a silicate bacterium |
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| Zhou Hong-bo , Zeng Xiao-xi , Liu Fei-fei , Qiu Guan-zhou and Hu Yue-hua.Screening, identification and desilication of a silicate bacterium[J].Journal of Central South University of Technology,2006,13(4):337-341. |
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| Authors: | Zhou Hong-bo Zeng Xiao-xi Liu Fei-fei Qiu Guan-zhou and Hu Yue-hua |
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| Affiliation: | (1) School of Minerals Processing and Bioengineering, Central South University, 410083 Changsha, China |
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| Abstract: | The strain Lv1–2 isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification,
and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite
concentration on the desilicating ability of the strain Lv1–2 were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick
capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv1–2 is similar to Bacillus mucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv1–2 and the B. mucilaginosus YNUCC0001 (AY571332) is more than 99%. Based on the above results, the strain Lv1–2 is identified as B. mucilaginosus. The optimum conditions for the strain Lv1–2 to remove silicon from illite are as follows: temperature is 30 °C; initial pH value is 7.5; medium volume in 200 mL bottle
is 60 mL; shaking speed of rotary shaker is 220 r/m; illite concentration is 1%.
Foundation item: Project (50321402) supported by the National Natural Science Foundation of China; project (2004CB619204) supported by the
National Basic Research Program |
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| Keywords: | screening identification Bacillus mucilaginosus silicon removal |
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