负载白芨多糖的PU/PAM双网络水凝胶的制备与性能

采用聚乙二醇、Ymer N120、聚丙二醇和异佛尔酮二异氰酸酯为原料,以三乙醇胺作交联剂合成聚氨酯(PU)预聚物,浸入白芨多糖(BSP)和丙烯酰胺(AM)混合溶液,通过自由基聚合制备了负载BSP的PU/PAM双网络水凝胶.采用FTIR、SEM对水凝胶的结构和形貌进行了表征,通过拉力试验机和生物实验对其力学性能和生物性能进行了测试.结果表明,当三乙醇胺用量为多元醇物质的量的60％时,双网络水凝胶具有高溶胀率(256％)的同时保持一定的拉伸强度(1.9 MPa)和高压缩强度(22.7 MPa).双网络水凝胶具有抗菌抗氧化作用,其中,双网络水凝胶对大肠杆菌和金黄色葡萄球菌的抑菌带宽度分别为0.5～4.0和0.5～3.5 mm,羟基自由基清除率最高为28％;溶血率低于5％,细胞存活率最高达101.3％±3.6％,表明双网络水凝胶具有良好的生物相容性.

Preparation of antibacterial and antioxidant double network hydrogel

Use polyethylene glycol (PEG), Ymer N120, poly propylene glycol (PPG) and isophorone diisocyanate (IPDI) to synthesize polyurethane (PU) prepolymer with triethanolamine. Triethanolamine was used as a cross-linking agent. Then the prepolymer was immersed in the mixed solution of bletilla striata polysaccharide (BSP) and acrylamide (AM) to prepare BSP-loaded PU/PAM double network hydrogel through free radical polymerization. The structural characteristics, mechanical properties and biological properties of the hydrogel were characterized by FTIR, SEM, tensile compression and biological tests. The results showed that when the amount of triethanolamine added is 60% (the mole fraction of polyol), the dual network hydrogel had a high swelling rate (203%) while maintaining a certain tensile strength (1.9 MPa) and high compressive strength (22.7 MPa). The double-network hydrogel had antibacterial and antioxidant effects. The antibacterial bandwidths of Escherichia coli were 0.5 mm~4 mm. And the antibacterial bandwidths of Staphylococcus aureus were 0.5 mm~3.5 mm. The scavenging rate of hydroxyl radical was up to 28%. The rate of hemolysis was less than 5 %. And the rate of cell survival was up to 101.3%. Those can indicate that the double network hydrogel had good biocompatibility.