高效毛细管电泳技术鉴别杏仁露中巴旦木掺假

目的 建立高效毛细管电泳法(high performance capillary electrophoresis, HPCE)鉴别杏仁露中巴旦木掺假的半定量鉴别方法。方法 样品经裂解液裂解, 超滤脱盐获得粗蛋白。粗蛋白经2-巯基乙醇还原, HPCE分离, 以特征峰与内标的迁移时间比值和特征峰峰强度差异进行定性和半定量分析。结果 经过优化的方法检测, 当植物蛋白饮料蛋白质含量为0.55 g/100 g时, 迁移时间比值1.16~1.19的电泳峰为单峰, 可判定仅含巴旦木蛋白; 当迁移时间比值1.16~1.19的电泳峰为双峰, 且前峰峰高高于后峰时, 可判定掺入了巴旦木, 判定限为10%。结论 本方法简单快速、操作性强, 适用于杏仁露中巴旦木的掺假鉴别。

Identification of adulteration of almond in apricot kernel beverage by high performance capillary electrophoresis

Objective To establish a semi quantitative identification method for adulteration of almond in apricot kernel beverage by high performance capillary electrophoresis (HPCE). Methods The sample was lysed by cellular lysate, and the crude protein was obtained by ultrafiltration desalination. The crude protein was reduced by 2-mercaptoethanol, and separated by HPCE, qualitative and semi-quantitative analysis were performed based on the migration time ratio between the characteristic peak and the internal standard and the difference in the characteristic peak intensities. Results Through the optimized method, when the protein content of the plant protein beverage was 0.55 g/100 g, the chromatographic peak with migration time ratio of 1.16?1.19 was single peak, which could be determined to contain only almond protein; when the electrophoretic peak with migration time ratio of 1.16?1.19 was double peak, and the forward peak height was higher than the back peak, it could be judged that the almond was mixed, and the judgment limit was 10%. Conclusion This method is simple, rapid and easy to operate, which is suitable for the adulteration identification of almond syrup.