基于Nafion/Hb/Fe3O4@Cs/GCE传感器检测油炸食品中丙烯酰胺

目的 制备Nafion/Hb/Fe3O4@Cs/GCE传感器, 并应用于油炸食品中丙烯酰胺(acrylamide, AM)的检测分析。方法 制备Fe3O4@碳球纳米复合材料(Fe3O4 carbon sphere nanocomposites, Fe3O4@Cs), 并复合Nafion和血红蛋白(hemoglobin, Hb), 以玻碳电极(glassy carbon electrode, GCE)为工作电极制备Nafion/Hb/Fe3O4@Cs/GCE传感器, 通过对固载方式、Hb固载量、加成温度以及加成时间的优化确定Nafion/Hb/Fe3O4@Cs/GCE对丙烯酰胺进行定量检测的最佳检测条件。结果 Fe3O4@Cs导电性良好, 能显著提高Nafion/Hb/Fe3O4@Cs/GCE传感器的灵敏度。AM与Hb的加成率与其浓度负对数在1×10?5~1×10?9 mol/L范围内呈良好的线性关系, 线性方程为Y=?5.254X+60.01 (X为AM浓度的负对数), r2=0.9973, 检出限为2.134×10?10 mol/L, 加标回收率在95.6%~98.4%之间, 相对标准偏差小于1.45%。结论 该方法简单、方便快捷, 适用于油炸食品中AM的定量分析。

Detection of acrylamide in fried food based on Nafion/Hb/Fe3O4@Cs/GCE sensor

Objective To prepare Nafion/Hb/Fe3O4@Cs/GCE sensor, and apply to the detection and analysis of acrylamide (AM) in fried food. Methods Fe3O4 carbon sphere nanocomposites (Fe3O4@Cs) was synthesized, and Nafion/Hb/Fe3O4@Cs/GCE sensor was prepared with Fe3O4@Cs combined with Nafion and hemoglobin (Hb) by using glassy carbon electrode (GCE) as working electrode, the optimum conditions that Nafion/Hb/Fe3O4@Cs/GCE detected acrylamide were determined by optimizing the loading method, Hb loading capacity, addition temperature and addition time. Results Fe3O4@Cs had a good conductivity and could significantly improve the sensitivity of the Nafion/Hb/Fe3O4@C/GCE sensor. There was a good linear relationship between the addition rate of AM and Hb and the negative logarithm of AM concentration in the range of 1×10?5-1×10?9 mol/L, the linear equation was Y=?5.254X+60.01 (X was the negative logarithm of AM concentration), r2=0.9973, the limit of detection was 2.134×10?10 mol/L, and the recovery rates were between 95.6% and 98.4%, and the relative standard deviation was less than 1.45%. Conclusion This method is simple and rapid, which is suitable for the quantitative analysis of acrylamide in fried food.