根内根孢囊霉对大豆成熟期主要生物量和福美双残留的影响

目的 探究接种根内根孢囊霉(Rhizophagus intraradices)菌剂对正茬与迎茬大豆成熟期主要生物量、根际土壤细菌和固氮菌菌落总数及大豆籽粒和根际土壤中福美双残留量的影响。方法 通过高效液相色谱法, 以甲醇和水为流动相, 使用C18色谱柱和紫外可变波长检测器于280 nm波长下测定大豆和根际土壤中福美双残留量, 分析R. intraradices对福美双残留量的影响。结果 接种R. intraradices菌剂后, 大豆主要生物量增加了18%~65%, 根际土壤细菌和固氮菌菌落总数增加了69%~154%; 喷洒福美双后, 大豆主要生物量增加了10%~34%, 根际土壤细菌和固氮菌菌落总数降低了73%~119%, 且福美双在大豆籽粒和土壤中产生残留; 接种R. intraradices+喷洒福美双(RF)处理组, 大豆籽粒和根际土壤中福美双残留量降低了24%~39%。结论 接种R. intraradices菌剂可以有效促进大豆作物生长, 提高大豆产量, 降解大豆籽粒及土壤中福美双残留。

Effects of Rhizophagus intraradices on the main biomass and thiram residues of soybean at maturity stage

Objective To study the effects of inoculation of Rhizophagus intraradices on the main biomass, the total number of colonies, the number of azotobacter in rhizosphere soil and the thiram residues in soybean grains and rhizosphere soil during the mature period of the continuous and alternate cropping soybean. Methods The residue of thiram in soybean and rhizosphere soil was determined by high performance liquid chromatography with methanol and water as mobile phase, C18 column and ulraviole variable wavelength detector at 280 nm, and the effects of R. intraradices on the residues of thiram was analyzed. Results After inoculating with R. intraradices, the main biomass of soybean had increased by 18%?65%, and the total number of bacteria and azotobacter in rhizosphere soil had increased by 69%?154%. After spraying thiram, the main biomass of soybean had increased by 10%?34%, the total number of bacteria and azotobacter in rhizosphere soil had decreased by 73%?119%, and thiram residues were produced in seeds and soil of soybean. After inoculating with R. intraradices and spraying thiram (RF), the residues of thiram in soybean seeds and rhizosphere soil had decreased by 24%?39%. Conclusion Inoculation with R. intraradices can effectively promote the growth of soybean crops, increase soybean yield and degrade thiram residues in seeds and soil of soybean.