Si-doping bone composite based on protein template-mediated assembly for enhancing bone regeneration |
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Authors: | Qin Yang Yingying Du Yifan Wang Zhiying Wang Jun Ma Jianglin Wang Shengmin Zhang |
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Affiliation: | 1.Advanced Biomaterials and Tissue Engineering Center,Huazhong University of Science and Technology,Wuhan,China;2.Department of Biomedical Engineering,Huazhong University of Science and Technology,Wuhan,China |
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Abstract: | Bio-inspired hybrid materials that contain organic and inorganic networks interpenetration at the molecular level have been a particular focus of interest on designing novel nanoscale composites. Here we firstly synthesized a series of hybrid bone composites, silicon-hydroxyapatites/silk fibroin/collagen, based on a specific molecular assembled strategy. Results of material characterization confirmed that silicate had been successfully doped into nano-hydroxyapatite lattice. In vitro evaluation at the cellular level clearly showed that these Si-doped composites were capable of promoting the adhesion and proliferation of rat mesenchymal stem cells (rMSCs), extremely enhancing osteoblastic differentiation of rMSCs compared with silicon-free composite. More interestingly, we found there was a critical point of silicon content in the composition on regulating multiple cell behaviors. In vivo animal evaluation further demonstrated that Si-doped composites enabled to significantly improve the repair of cranial bone defect. Consequently, our current work not only suggests fabricating a potential bone repair materials by integrating element-doping and molecular assembled strategy in one system, but also paves a new way for constructing multi-functional composite materials in the future. |
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