| 酶解丝素蛋白制备ACE抑制肽的研究 |
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| 引用本文： | 吴晖,罗美琪,唐语谦,肖性龙,袁坤,李晓凤. 酶解丝素蛋白制备ACE抑制肽的研究[J]. 现代食品科技, 2011, 27(12): 1461-1465 |
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| 作者姓名： | 吴晖 罗美琪 唐语谦 肖性龙 袁坤 李晓凤 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东广州,510561 |
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| 基金项目： | 国家自然科学基金，新世纪优秀人才支持计划项目，高校博士点基金 |
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| 摘 要： | 本研究首次探讨利用Alcalase酶解丝素蛋白制备高活性ACE抑制肽.以水解度为主要评价指标,研究了温度、浓度、pH、加酶量以及反应时间对酶解反应的影响.分析了不同水解度下的酶解产物与ACE体外抑制活性、肽得率和蛋白回收率的关系.确定了制备ACE抑制肽的最佳酶解反应DH值为17.12％,此时所得丝素蛋白肽的肽得率为50...
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| 关 键 词： | 丝素蛋白 ACE抑制活性 活性肽 |
Enzymatic Hydrolysis of Silk Fibroin for Production of ACE Inhibitors |
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| WU Hui,LUO Mei-qi,TANG Yu-qian,XIAO Xing-long,Yuan Kun,Li Xiao-feng. Enzymatic Hydrolysis of Silk Fibroin for Production of ACE Inhibitors[J]. Modern Food Science & Technology, 2011, 27(12): 1461-1465 |
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| Authors: | WU Hui LUO Mei-qi TANG Yu-qian XIAO Xing-long Yuan Kun Li Xiao-feng |
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| Affiliation: | (College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510561,China) |
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| Abstract: | Silk fibroin was hydrolyzed by Alcalase to produce the peptide with high ACE-inhibition activity.The effects of several influential factors on the enzymatic hydrolysis were investigated and the optimum reaction conditions were determined as follows: pH 8.5,temperature 60 ℃,substrate concentration 5%,enzyme dosage 1000 U/g protein,and reaction time 240 min,under which the DH,ACE-inhibition rate,peptide yield and protein recovery of the product were17.12%,74.07%,50.31% and 77.37%,respectively.MALDI-TOF mass spectrometry of the hydrolysate showed that the molecular weight of the peptides was below 2.4kDa. |
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| Keywords: | silk fibroin ACE inhibitory activity active peptides |
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