波叶山蚂蝗总黄酮提取工艺研究

研究了波叶山蚂蝗(Desmodium sequax Wall)总黄酮的最佳提取工艺。利用单因素实验研究了乙醇回流、超声波两种提取法对波叶山蚂蝗中总黄酮提取率的影响,用紫外分光光度法进行含量测定。结果表明:乙醇回流提取提取法的最佳工艺条件为:提取温度70℃,料液比1∶50 g/mL,乙醇浓度60%,提取时间2.0 h,提取率为2.209%;超声波提取法的最佳工艺条件为:1∶20 g/mL的料液比,70%的乙醇浓度,提取时间40 min,提取率为1.256%。结论乙醇回流提取法的效果较为优越,其提取率远远大于超声波提取法。

Optimization of Extraction Process for Total Flavonoids from Desmodium sequax Wall

Optimal extraction parameters of total flavonoids from Desmodium sequax Wall was studied.The effects of two methods including alcohol reflux and Ultrasonic wave on extraction efficiency were investigated by one-factor-at-a-time and the ultraviolet spectrophotometric method was used to determine the content.The results indicated that the optimum conditions of alcohol reflux were as follows： extraction temperature 70 ℃,material/liquid ratio 1：50 g/mL,alcohol concentration 60%,extraction time 2.0 h.The extraction rate was 2.209%,and the optimum conditions of Ultrasonic wave were as below： material/liquid ratio 1：20 g/mL,alcohol concentration 70%,extraction time 40 min.The extraction rate was 1.256%.Alcohol refluxing extraction effect was more superior,and the extraction yield was far greater than Ultrasonic wave.