MnO2/ZnO/GCE电化学传感器的构建及对蜂蜜中磺胺甲恶唑的检测

为快速检测蜂蜜中磺胺甲恶唑(sulfamethoxazole,SMZ)的残留，将水热法制备的氧化锌(ZnO)及共沉淀法制备的二氧化锰(MnO₂)材料复合于玻碳电极(GCE)上，构建了MnO₂/ZnO/GCE电化学传感器用于检测SMZ。采用扫描电镜(SEM)及红外光谱(FT-IR)对复合材料的形貌、结构进行表征。利用差分脉冲伏安法(DPV)对复合材料的比例、修饰量进行探究，从而对传感器的构建条件进行优化。并采用差分脉冲伏安法(DPV)及循环伏安法(CV)对缓冲溶液pH、扫描速率等检测条件进行考察。结果表明，当m(ZnO):m(MnO₂)质量比为1:0.6、MnO₂/ZnO复合材料修饰量为1.7×10-3 mg/mm2、缓冲溶液pH为8、扫描速率为30 mV/s时，该传感器对SMZ具有良好的检测效果。在0.3～100μmol/L范围内，峰电流与SMZ浓度呈良好的线性关系，其线性方程为Ip=0.0843 c+4.5168(R2=0.9916)，检测限为0.39μmol/L，定量限为1.30μmol/L。将...

Construction of MnO2/ZnO/GCE Electrochemical Sensor and the Detection of Sulfamethoxazole in Honey

In order to rapidly detect sulfamethoxazole (SMZ) residues in honey, zinc oxide (ZnO) prepared by hydrothermal method and manganese dioxide (MnO₂) prepared by co-precipitation method were compounded on glassy carbon electrode (GCE) to construct MnO₂/ZnO/GCE electrochemical sensor for the detection of SMZ. The morphology and structure of the composites were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Differential pulse voltammetry (DPV) was used to explore the ratio and modification amount of the composite material to optimize the construction conditions of the sensor. DPV and cyclic voltammetry (CV) were used to investigate the detection conditions such as buffer solution pH and scanning rate. The results showed that when the mass ratio of m(ZnO):m(MnO₂) was 1:0.6, the addition amount of MnO₂/ZnO composite was 1.7×10?3 mg/mm2, the pH was 8 and the scanning rate was 30 mV/s, the sensor had a good detection effect on SMZ. In the range of 0.3~100 μmol/L, the peak current of SMZ had a good linear relationship with the concentration, the linear equation was I_p=0.0843c+4.5168 (R2=0.9916), the limit of detection was 0.39 μmol/L and the limit of quantification was 1.30 μmol/L. The sensor was applied to the determination of honey sample, with a recovery of 95.5%~102.6% and a relative standard deviation (RSD) of 0.68%~2.55%. It shows that the sensor can realize the accurate detection of SMZ content. It has certain application value for the detection of SMZ residues in honey samples.