植物乳杆菌AR495通过降低关节组织炎症水平改善大鼠骨关节炎

植物乳杆菌AR495(Lactobacillus plantarum AR495)具有良好的抗炎、缓解骨质疏松作用。为进一步探讨L. plantarum AR495缓解骨关节炎（osteoarthritis,OA）的效果和作用机制,本实验建立了OA大鼠模型,考察L. plantarum AR495对OA大鼠骨关节炎标志物、炎症因子水平以及氧化应激损伤等指标的影响,并通过关节部位病理学染色和Micro-CT检测以验证软骨基质和骨质恢复情况。结果显示,L. plantarum AR495能够显著缓解OA大鼠因活动量减少而导致的体质量过度增长（P<0.05）;病理分析表明,L. plantarum AR495能够显著降低OA大鼠关节组织中炎症因子肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平（P<0.05）,抑制金属基质蛋白酶-13(matrix metalloproteinase-13,MMP-13)和软骨基质降解物II型胶原C端肽（C-telopeptideoftypeIIcollagen,CTX-II）的生成（P<0.05）,从而缓解...

Lactobacillus plantarum AR495 Improves Osteoarthritis in Rats by Reducing the Level of Joint Inflammation

Previous studies have shown that Lactobacillus plantarum AR495 has good anti-inflammatory and anti-osteoporosis effects. In order to further explore the effect and mechanism of AR495 in alleviating osteoporosis (OA), a rat model of OA was established to investigate the effects of AR495 on OA markers, inflammatory factor levels and oxidative stress injury in OA rats and to verify the recovery of cartilage matrix and bone by pathological staining of joints and microtomography (micro-CT) imaging. The results showed that AR495 could significantly alleviate excessive body mass gain caused by decreased physical activity in OA rats (P < 0.05). Pathological analysis showed that AR495 could significantly reduce the level of tumor necrosis factor-α (TNF-α) in the joint tissue of OA rats, inhibit the production of matrix metalloproteinase-13 (MMP-13) and the production of C-telopeptide of type II collagen (CTX-II) as a degradation product of cartilage matrix, thereby alleviating the degradation of articular cartilage matrix in rats. Micro-CT imaging showed that AR495 could significantly alleviate bone loss in the joint of OA rats and effectively protect the integrity of cartilage and synovium of rats. In addition, AR495 could significantly increase the activity of superoxide dismutase (SOD) and reduce the concentration of malondialdehyde (MDA) in peripheral blood (P < 0.05). Quantitative real-time polymerase chain reaction (qPCR) analysis showed that AR495 could stimulate the expression of the mucin-2 (MUC-2) and occludin-1 genes in intestinal goblet cells and repair the intestinal barrier function. It can be seen that AR495 can alleviate OA inflammation level and create a beneficial internal environment for chondrocytes to exert their functions and produce collagen matrix, thereby alleviating and restoring joint injury and improving OA symptoms.