超高效液相色谱-稳定性同位素稀释质谱法测定预包装食品中不同阶段美拉德反应产物含量

建立基于稳定同位素内标稀释的超高效液相色谱-串联三重四极杆质谱同步检测预包装食品中羧甲基赖氨酸(N^ε-(carboxymethyl)-lysine,CML)、羧乙基赖氨酸(N^ε-(carboxyethyl)-lysine,CEL)、甲基乙二醛-羟基咪唑酮异构体(methyglyoxal-derived hydroimidazolones,MG-H1、MG-H2、MG-H3)、乙二醛-氢咪唑酮(glyoxal-derived hydroimidazolone,G-H1)、糠氨酸7种不同阶段美拉德反应产物(Maillard reaction products,MRPs)的含量。采用Kinetex C₁₈色谱柱进行分离，多反应监测模式进行定量分析。该方法的加标回收率为88.4%～111.3%，精密度相对标准偏差小于9%，检出限和定量限分别为2.1～14.2 ng/mL和7.4～41.2 ng/mL。采用该方法对134种常见预包装食品中的7种MRPs进行定量，并对得到的数据进行主成分分析和相关性分析。结果表明，CML、MG-H1...

Detection of Maillard Reaction Products at Different Stages in Prepackaged Foods by Ultra-high Performance Liquid Chromatography-Stable Isotope Dilution Mass Spectrometry

A method was developed for the simultaneous determination of seven Maillard reaction products (MRPs), namely Nε-(carboxymethyl)-lysine (CML), Nε-(carboxyethyl)-lysine (CEL), methyglyoxal-derived hydroimidazolones (MG-H1, MG-H2 and MG-H3), glyoxal-derived hydroimidazolone (G-H1) and furosine in prepackaged foods by ultra-high performance liquid chromatography-triple quadrupole-tandem mass spectrometry (UPLC-QqQ-MS/MS). The chromatographic separation was performed on a Kinetex C18 column and quantification was performed in the multiple reaction monitoring (MRM) mode. The recoveries of the method ranged from 88.4% to 111.3% with relative standard deviations (RSDs) less than 9% in terms of precision, and the limit of detection and the limit of quantification were 2.1–14.2 and 7.4–41.2 ng/mL, respectively. The developed method was applied to 134 typical prepackaged foods, and the obtained data was analyzed by principal component analysis (PCA) and correlation analysis (CA). The results showed that CML, MG-H1/3, MG-H2 and G-H1 were the major MRPs in prepackaged foods, and they had significant positive correlations with each other and with protein, fat and total sugar contents. This method shows acceptable linearity, accuracy and precision and is suitable for the rapid determination of multiple MRPs in prepackaged foods.