基于Cu,Fe-N-C氧化酶活性构建邻苯二甲酸酯荧光检测探针

基于邻苯二甲酸酯（phthalic acid ester,PAEs）对双金属原子Cu,Fe-N-C纳米酶模拟氧化酶活性的调节，建立简单、灵敏测定发酵食品中PAEs的荧光探针方法。PAEs吸附于Cu,Fe-N-C表面后，会加速Cu,Fe-N-C催化氧化底物邻苯二胺，使生成的2,3-二氨基吩嗪产物荧光强度增加，PAEs在1.25～50μg/L范围内呈良好的线性关系。将该研究方法用于PAEs含量的测定，其检出限可达到0.76μg/L，实际样品的加标回收率在88.0%～104.6%范围内，满足GB 5009.271—2016中的检测要求。该方法具有简便、稳定、快速等特点，其检出限也低于国家规定的食品中PAEs含量的限值。

A Fluorescent Probe Based on Cu,Fe-N-C Nanozyme with Oxidase-like Activity for Detection of Phthalate Esters

A simple and sensitive fluorescent probe for the determination of phalic acid ester (PAEs) in fermented foods was developed based on the fact that the oxidase-like activity of Cu,Fe-N-C nanozyme can be regulated by PAEs. After PAEs were adsorbed on the surface of Cu,Fe-N-C, the catalytic oxidation of the substrate o-phenylenediamine (OPD) was accelerated, thus increasing the fluorescence intensity of the generated 2,3-diaminophenazine (DAP). The calibration curve for PAEs showed a good linear relationship in the concentration range of 1.25–50 μg/L. The detection limit of the proposed method was 0.76 μg/L. The recoveries of spiked samples ranged from 88.0% to 104.6%, which meet the detection requirements of the national standard GB 5009.271-2016. This method is simple, stable and rapid, and its detection limit is lower than the national limit for PAEs content in foods.