Lactococcus lactis subsp. lactis HFY14对狼疮性肾炎小鼠肾功能的改善作用

本实验研究分离自四川红原自然发酵牦牛酸乳的Lactococcus lactis subsp. lactis HFY14(LLSLHFY14)对降植烷诱导狼疮性肾炎小鼠肾功能改善的效果。采用不同剂量LLSLHFY14灌胃小鼠，分析小鼠血清白细胞介素(interleukin,IL)-2、IL-12等免疫指标的变化情况，然后通过苏木精-伊红(hematoxylin-eosin,H&E)切片观察肾脏组织的病变情况，通过定量聚合酶链式反应(quantitative polymerase chain reaction,qPCR)和Western blot测定肾脏组织中的IL-4、IL-1β和转化生长因子-β(transforming growth factor beta,TGF-β)mRNA相对表达量和蛋白相对表达量。结果显示，LLSLHFY14能够提升狼疮性肾炎小鼠胸腺指数和脾脏指数。血清实验显示LLSLHFY14能够抑制狼疮性肾炎造成的小鼠IL-2、IL-12、总蛋白(total protein,TP)、白蛋白(albumin,ALB)质量浓度下降和干扰素γ(interferon ga...

Lactococcus lactis subsp. lactis HFY14 Improves Renal Function in Mice with Lupus Nephritis

In this study, the effect of Lactococcus lactis subsp. lactis HFY14 (LLSLHFY14) isolated from naturally fermented yak yoghurt in Hongyuan county of Sichuan in improving renal function in mice with lupus nephritis induced by norphytane was investigated. The effects of different doses of LLSLHFY14 on immune indexes such as interleukin (IL)-2 and IL-12 were measured. Then, renal histopathology was observed by hematoxylin-eosin (H&E) staining, and the relative mRNA and protein expression of IL-4, IL-1β and transforming growth factor beta (TGF-β) in renal tissue were measured by quantitative polymerase chain reaction (qPCR) and Western blot. The results showed that LLSLHFY14 could increase thymus and spleen indexes in mice with lupus nephritis. LLSLHFY14 could inhibit the decrease in IL-2, IL-12, total protein (TP) and albumin (ALB) and the increase in interferon gamma (IFN-γ), transforming growth factor beta (TGF-β), immunoglobulin G (IgG), serum creatinine (SCr), blood urea nitrogen (BUN), total cholesterol (TC) and triglyceride (TG) in the serum of mice with lupus nephritis. LLSLHFY14 could also reduce the positive rate of anti-double stranded deoxyribonucleic acid (dsDNA) antibodies in mice caused by norphytane. Pathological examination showed that LLSLHFY14 could restore glomerular morphologic integrity and inflammatory infiltration in mice with lupus nephritis. Furthermore, LLSLHFY14 could up-regulate the expression of IL-4 and down-regulate the expression of IL-1β and TGF-β in the kidney. It can be concluded that LLSLHFY14 significantly improves renal function in mice with lupus nephritis in a positive concentration-dependent way. The effect of LLSLHFY14 at high concentration (109 CFU/kg) is close to that of prednisone. This study lays a foundation for further utilization of lactic acid bacteria in naturally fermented yak yogurt.