| 鹰嘴豆多糖分离纯化和结构表征 |
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| 引用本文： | 赵佳莹,辛玥,宋萧萧,聂少平,殷军艺.鹰嘴豆多糖分离纯化和结构表征[J].食品科学,2023,44(8):40-45. |
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| 作者姓名： | 赵佳莹 辛玥 宋萧萧 聂少平 殷军艺 |
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| 作者单位： | （南昌大学 食品科学与技术国家重点实验室，中国-加拿大食品学与技术联合实验室（南昌），江西省生物活性多糖重点实验室，江西 南昌 330047） |
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| 基金项目： | “十三五”国家重点研发计划重点专项（2018YFE0108300） |
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| 摘 要： | 以鹰嘴豆为原料，对其全豆、子叶、种皮等不同部位多糖分布和理化性质进行分析、比较，并研究鹰嘴豆种皮多糖(Cicer arietinum hull polysaccharide,CAHP)结构特征。根据得率和化学组成等结果，可推测鹰嘴豆多糖集中分布于其种皮部位；进一步采用柱层析分离法对CAHP纯化获得6个组分，并对其中得率较高且具有良好均一性的多糖组分CAHP-F0.2进行结构表征。结果表明，CAHP-F0.2以HG型果胶多糖为主，其主链由1,4-Galp A连接而成，并含有少量的RG-I型果胶结构域，同时存在阿拉伯聚糖侧链和阿拉伯半乳聚糖-II侧链。本研究将为鹰嘴豆多糖结构、生物活性研究和资源开发提供一定理论依据。
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| 关 键 词： | 鹰嘴豆 多糖 分离纯化 结构特征 |
Isolation,Purification and Structural Characterization of Polysaccharides from Chickpea |
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| ZHAO Jiaying,XIN Yue,SONG Xiaoxiao,NIE Shaoping,YIN Junyi.Isolation,Purification and Structural Characterization of Polysaccharides from Chickpea[J].Food Science,2023,44(8):40-45. |
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| Authors: | ZHAO Jiaying XIN Yue SONG Xiaoxiao NIE Shaoping YIN Junyi |
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| Affiliation: | (State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang 330047, China) |
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| Abstract: | In this research, the distribution and physicochemical properties of polysaccharides from the whole seed, cotyledon and hull of Cicer arietinum were analyzed and compared, and the structure characteristics of Cicer arietinum hull polysaccharide (CAHP) were characterized. It was found that polysaccharide was concentrated in the hull of chickpea according to the yield and composition of polysaccharide. CAHP was further purified by column chromatography into six fractions (Fw, F0.1, F0.2, F0.3, F0.5 and F2), and the structure of CAHP-F0.2, which showed relatively high yield and good homogeneity, was characterized. The results showed that CAHP-F0.2 was consisted of mostly homogalacturonan (HG)-type pectic polysaccharide whose main chain was linked by 1,4-GalpA, and contained a small amount of RG-I domain, with arabincan and arabinogalactan-II side chain. This study will provide a theoretical basis for future research on the structure, biological activity and resource development of chickpea polysaccharides. |
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| Keywords: | chickpea polysaccharide separation and purification structural characteristics |
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