| 牛骨肽钙螯合物制备、表征及其促MC3T3-E1细胞成骨活性 |
| |
| 引用本文： | 齐立伟,张鸿儒,郭玉杰,刘泓,李瑞林,张春晖,徐杨.牛骨肽钙螯合物制备、表征及其促MC3T3-E1细胞成骨活性[J].食品科学,2023,44(6):107-115. |
| |
| 作者姓名： | 齐立伟 张鸿儒 郭玉杰 刘泓 李瑞林 张春晖 徐杨 |
| |
| 作者单位： | （1.中国农业科学院农产品加工研究所，农业农村部农产品加工综合性重点实验室，北京 100193；2.宁夏大学食品与葡萄酒学院，宁夏 银川 750021；3.内蒙古蒙肽生物工程有限公司，内蒙古 呼和浩特 011517） |
| |
| 基金项目： | 内蒙古自治区科技计划技术攻关类“揭榜挂帅”项目（2022JBGS0007）；
国家农业科技创新工程项目（CAAS-ASTIP-2022-IFST） |
| |
| 摘 要： | 目的：为制备人体高效吸收利用的补钙剂并探究其对MC3T3-E1细胞成骨活性的影响。方法：对牛骨多肽进行钙螯合处理，以钙螯合能力为考察指标，通过响应面方法确定最佳制备条件；利用红外光谱、X射线衍射和原子吸收等方法探究肽钙螯合物的结构特性及其稳定性；通过细胞实验探究牛骨肽钙螯合物对MC3T3-E1细胞增殖、分化和矿化活性的影响。结果：制备牛骨肽钙螯合物的最佳条件为肽钙质量比2.97∶1、温度62.54℃、pH 9.06。在该条件下，钙螯合能力达到55.65 μg/mg。光谱结构分析结果表明氨基氮、羟基氧和羧基氧是钙主要螯合位点，二者之间主要通过配位键结合，且肽螯合钙后分子质量增加；此外，牛骨肽钙螯合物稳定性受温度影响较小，但对pH值较敏感。MC3T3-E1细胞实验结果表明肽钙螯合物具有明显促MC3T3-E1细胞增殖、分化和矿化活性的作用。结论：制备的牛骨肽钙螯合物具有潜在的促MC3T3-E1细胞成骨活性功效。
|
| 关 键 词： | 牛骨肽钙螯合物 结构表征 MC3T3-E1细胞 成骨活性 |
Preparation and Characterization of Bovine Bone Peptide-Calcium Chelate and Its Promoting Effect on Osteogenic Activity of MC3T3-E1 Cells |
| |
| QI Liwei,ZHANG Hongru,GUO Yujie,LIU Hong,LI Ruilin,ZHANG Chunhui,XU Yang.Preparation and Characterization of Bovine Bone Peptide-Calcium Chelate and Its Promoting Effect on Osteogenic Activity of MC3T3-E1 Cells[J].Food Science,2023,44(6):107-115. |
| |
| Authors: | QI Liwei ZHANG Hongru GUO Yujie LIU Hong LI Ruilin ZHANG Chunhui XU Yang |
| |
| Affiliation: | (1. Comprehensive Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. School of Food and Wine, Ningxia University, Yinchuan 750021, China; 3. Inner Mongolia Mengtai Biological Engineering Co. Ltd., Hohhot 011517, China) |
| |
| Abstract: | Purpose: To prepare a calcium supplement for efficient absorption and utilization in the human body and to explore its effect on the osteogenic activity of MC3T3-E1 cells. Methods: Bovine bone peptide was chelated with calcium, and the optimal reaction conditions were determined by response surface methodology (RSM). The structural characteristics and stability of peptide-calcium chelate were studied by infrared spectroscopy, X-ray diffraction and atomic absorption spectrometry. The effects of bovine bone peptide-calcium chelate on the proliferation, differentiation and mineralization of MC3T3-E1 cells were investigated. Results: The optimum conditions for the preparation of bovine bone peptide calcium chelate were peptide/calcium mass ratio 2.97, temperature 62.54 ℃ and pH 9.06. Under these conditions, the calcium chelating capacity was 55.65 μg/mg. The results of spectral analysis showed that amino nitrogen, hydroxyl oxygen and carboxyl oxygen were the major calcium-chelating sites. Bovine bone peptide could form coordinate bonds with calcium, and the molecular mass of the peptide increased after chelation with calcium. In addition, the stability of bovine bone peptide-calcium chelate was little affected by temperature, but sensitive to pH. The peptide-calcium chelate could significantly promote the proliferation, differentiation and mineralization of MC3T3-E1 cells. Conclusion: Bovine bone peptide-calcium chelate has the potential to promote the osteogenic activity of MC3T3-E1 cells. |
| |
| Keywords: | bovine bone peptide-calcium chelate structural characterization MC3T3-E1 cells osteogenic activity |
|
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
