| 中空纤维超滤和疏水层析大规模纯化质粒DNA |
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| 引用本文： | 殷玉和,孙博,郭丽姝,所丹,孔维.中空纤维超滤和疏水层析大规模纯化质粒DNA[J].粉末涂料与涂装,2007,20(2):133-135. |
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| 作者姓名： | 殷玉和 孙博 郭丽姝 所丹 孔维 |
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| 作者单位： | 吉林大学生命科学院 长春130012(殷玉和，孙博，孔维)，长春百克药业有限公司质量部 长春130012(郭丽姝)，长春长生基因有限公司生产部 长春130012(所丹) |
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| 摘 要： | 目的建立适合大规模纯化质粒DNA的工艺。方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。结果纯化后的质粒DNA纯度达95%以上,整个工艺流程总产率为58%,各项指标均符合药用质粒DNA质量标准。结论此纯化工艺简便,产率较高,为DNA疫苗大规模纯化奠定了基础。
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| 关 键 词： | 质粒 超滤 疏水层析 |
| 文章编号： | 1004-5503（2007）02-133-03 |
| 收稿时间： | 2006-03-24 |
| 修稿时间： | 2006年3月24日 |
Large-scale Purification of Plasmid DNA by Ultrafiltration with Hollow Fiber Filter and Hydrophobic Chromatography |
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| YIN Yu-he , SUN Bo, GUO Li-shu, et al.Large-scale Purification of Plasmid DNA by Ultrafiltration with Hollow Fiber Filter and Hydrophobic Chromatography[J].Chinese Journal of Biologicals,2007,20(2):133-135. |
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| Authors: | YIN Yu-he SUN Bo GUO Li-shu |
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| Affiliation: | College of Life Science, Jilin University, Changchun 130012, China |
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| Abstract: | Objective To develop a procedure for large-scale purification of plasmid DNA.Methods Purify plasmid DNA by a procedure consisting of alkaline lysis,ultrafiltration with hollow fiber filter,hydrophobic chromatography and molecular sieve chromatography.Results The purified plasmid DNA reached a purity of more than 95% and met the requirements for plasmid DNA for medical use.The yield of the procedure was 58%.Conclusion The developed purification procedure was simple and of high yield.It laid a foundation of large-scale purification of DNA vaccine. |
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| Keywords: | Plasmid DNA Ultrafiltration Hydrophobic chromatography |
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