脯氨酸对肾上腺素电氧化反应的影响

采用循环伏安法和交流阻抗法,在铂电极上研究了脯氨酸对肾上腺素电氧化反应的影响。并用密度泛函理论的B3LYP方法,在6-31++G(2d)基组水平上对复合物进行结构优化。实验结果表明,脯氨酸对肾上腺素有一定的稳定作用,抑制肾上腺素的氧化,在循环伏安曲线上表现为氧化峰电位正移还原峰电位负移,峰电流减小;在阻抗谱中表现出电氧化反应的电阻增大。计算结果证明脯氨酸与肾上腺素形成了氢键复合物,且复合物中的氢键作用较强。

Influence of proline on electrochemical oxidation reaction of adrenaline

The influence of L-proline on the electrochemical oxidation of adrenaline was investigated by cyclic voltammetry and electrochemical impedance spectroscopy at a platinum electrode.Simultaneously the structure of complexes of protonated adrenaline with proline is optimized by means of the density function theory method at B3LYP/6-31++G（2 d）level.The experimental results show that proline has stabilization on adrenaline and to some extent inhibits the oxidation of adrenaline.Cyclic voltammetry display that oxidation peak potential shift positively,the reduction peak potential to shift negatively,peak currents decreased.Nyquist plot showed that the electrochemical reaction resistance is accreted.The calculation results indicated that stronger hydrogen bond complexes were formed by the interaction between adrenaline and proline.