Liaison of phenolic acids and biological activity of escalating cultivars of Daucus carota |
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Authors: | Nadeem Abbas Faisal Abdullah Ijaz Hussain Muhammad Ikram Shazia Anwer Bukhari |
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Affiliation: | 1. Natural Product and Synthetic Chemistry Lab, Department of Applied Chemistry and Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan;2. Vegetable Research, Ayub Agriculture Research Institute, Faisalabad, Pakistan |
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Abstract: | The objective of the present study was to analyze and compare the phenolic compounds and their antioxidant capacities of new lines of Dacus carota. The selected cultivars showed high variation in the contents of total phenolics (30.26–65.39 mg/100 g FW) and total ascorbic acid (41.12–58.36 mg/100 g FW). Analysis on RP-HPLC revealed that hydroxycinnamic acids and its derivatives were major phenolic compounds present in D. carota extracts, whereas 5-caffeolquinic acid was a major hydroxycinnamic acid (ranged from 30.26 to 65.39 mg/100 g FW). DCP cultivar showed high total antioxidant capacity (77.69 mg/100 g), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity (52.36 mg/100 g), superoxide radical scavenging capacity (53.69 mg/100 g), and hydroxyl radical scavenging capacity (51.91 mg/100 g). A linear relationship was found between total phenolic acid contents and antioxidant capacity. Both phenolic compounds and antioxidant capacities varied significantly (ρ < 0.05) among cultivars. DCP cultivar was found to be a rich source of phenolics and ascorbic acid with high antioxidant activity. |
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Keywords: | Antioxidant Free radicals HPLC Phenolics Scavenging capacity |
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