木醋杆菌发酵培养基优化及发酵方式的探讨

以木醋杆菌（Acetobacter xylinum）为出发菌株，细菌纤维素产量为指标，通过正交试验优化发酵培养基，其最优组成为葡萄糖15g/L、蛋白胨10g／L、酵母膏5g／L、玉米浆40mL/L、磷酸三钠3g／L、柠檬酸钠1g／L、硫酸镁2g／L、氯化钙0．2g／L、硫酸亚铁0．03g／L、乙醇10mL/L。适宜的发酵方式为先130r／min振荡培养10h，后静置培养10d。经验证试验，细菌纤维素产量可达3．81g／L。

Medium optimization and fermentation condition of Acetobacter xylinum

Using Acetobacter xylinum as primary strain,and bacterial cellulose yield as the index,the optimum component proportion was achieved by orthogonal experiment as: glucose 15 g/L,peptone 10 g/L,yeast extract 5 g/L,cream style corn 40 ml/L,Na3PO4 3 g/L,natrium of citrate 2 g/L,MgSO4 2 g/L,CaCl2 0.2 g/L,FeSO4 0.03 g/L,and ethanol 10 mL/L.The optimum fermentation condition was: first shaking culture for 10 hours,and then static cultivation for 10 days.The yield of bacterial cellulose could reach 3.81 g/L.