GC-QqQ-MS/MS法检测油脂类功能食品中辐照标志物2-十二烷基环丁酮和2-十四烷基环丁酮

建立了气相色谱-三重四极杆串联质谱（GC-QqQ-MS/MS）同时检测油脂类功能食品中辐照标志物-2-烷-基环丁酮（2-ACBs）类化合物中2-十二烷基环丁酮（2-DCB）和2-十四烷基环丁酮（2-TCB）的方法。样品用正己烷溶解，过ProElut Silica固相萃取柱净化。结果表明，2-DCB和2-TCB与基质干扰峰得到较好的分离，在0.005~1.0 mg/L浓度范围内，峰面积与浓度呈良好的线性关系，线性相关系数（R²）大于0.999。在油脂类功能食品中添加0.01、0.05、0.10 mg/kg水平的2-DCB和2-TCB，平均加标回收率为85.6%~97.1%，相对标准偏差（RSD）小于7.0%（n=6），方法定量限（LOQ）为5 μg/kg。该方法操作简便、耗时短、灵敏度高、稳定性好，能为油脂类功能食品中辐照标志物的检测提供理论依据。

Determination of 2-Dodecylcyclobutanone and 2-Tetradecylcyclobutanone in Irradiated Fat-Containing Function Food by GC-QqQ-MS/MS

A method for simultaneous detection of 2-alkylcyclobutanones was established by gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS/MS). Samples were dissolved by n-hexane, purified by ProElut Silica extraction column. The results show that 2-dodecylcyclobutanone(2-DCB), 2-tetradecylcyclobutanone(2-TCB) are well separated from matrix interference peaks. The peak area against concentration shows good linear relationship in the concentration of 0.005-1.0 mg/L, the linear correlation coefficients (R²) are greater than 0.999. Addition of 0.01, 0.05 and 0.10 mg/kg levels of 2-DCB and 2-TCB in fat-containing function food products, the average recovery ranges from 85.6% to 97.1%, the relative standard deviations (RSDs) are less than 7.0 % (n=6), and the limit of quantification is 5 μg/kg. The method is simple, time-saving, high sensitivity and good stability for real samples analysis，which can provide a theoretical basis for the detection of irradiated markers in the fat-containing function food.