利用质谱技术解析糖类结构的研究进展

糖类物质是重要的生物信息分子，在许多生命活动中发挥着重要作用。糖类物质的结构非常复杂，其结构解析一直是糖生物学研究的瓶颈。在糖类物质结构解析的诸多方法中，质谱技术被认为是一种不可缺少的重要手段。本工作综述了质谱及色谱-质谱联用法在糖类结构解析中的研究进展，包括电子轰击质谱、化学电离质谱、快原子轰击质谱、电喷雾质谱、基质辅助激光解析电离质谱、色谱-质谱联用技术(气相色谱-质谱和液相色谱-质谱)，并比较了各种方法在糖类物质定性、定量分析中的优缺点。随着质谱技术的不断发展，糖链的释放和衍生化方法的不断改进，以及谱图解析工作的进一步深入，质谱技术必将成为糖类物质结构解析的有力工具。

Research Progress on the Structure Analysis of Saccharides by Mass Spectrometry Technology

As a group of bioinformation molecules, saccharides play significant roles in many life activities. Due to their structural complexity, structural analysis of saccharides is the bottleneck of glycobiology research. In many structural analysis methods of saccharides, mass spectrometry technology is considered as an important, indispensable way. This paper summarized the advances in study on the structure analysis of saccharides by mass spectrometry and chromatography coupled with mass spectrometry. The methods of electron impact mass spectrometry, chemical ionization mass spectrometry, fast atom bombardment mass spectrometry, electro-spray ionization mass spectrometry, matrix-assisted laser desorption mass spectrometry and chromatography coupled with mass spectrometry were presented. This paper compared the advantages and disadvantages in the qualitative and qualitative analysis of saccharides. With the development of mass spectrometry techniques, the improvement of glycans release and derivatization method and further spectral analysis work, mass spectrometry technology will become a powerful tool for structure analysis of saccharides.