| 苦瓜多肽的分离纯化研究 |
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| 引用本文： | 高续春,代宏哲. 苦瓜多肽的分离纯化研究[J]. 当代化工, 2011, 40(5): 467-468 |
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| 作者姓名： | 高续春 代宏哲 |
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| 作者单位： | 陕西省榆林学院,化学与化工学院,陕西,榆林,719000 |
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| 摘 要： | 以苦瓜为原料分离纯化制备多肽,通过正交试验确定了酶解的最佳工艺条件:加酶量为4%,温度45 ℃,酶解时间为1 h,料液比为2,多肽得率提高19.7%.采用葡聚糖凝胶G-50纯化得到2组洗脱液.此分离纯化工艺简单可行,成本低,可适用于工业化生产.
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| 关 键 词： | 苦瓜 多肽 分离 纤维素酶 |
Study on Separation and Purification of Polypeptide From Momordica Charantia |
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| GAO Xu-chun,DAI Hong-zhe. Study on Separation and Purification of Polypeptide From Momordica Charantia[J]. Contemporary Chemical Industry, 2011, 40(5): 467-468 |
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| Authors: | GAO Xu-chun DAI Hong-zhe |
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| Affiliation: | GAO Xu-chun,DAI Hong-zhe(School of Chemistry and Chemical Engineering,Yulin University,Shanxi Yulin 719000,China) |
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| Abstract: | Momordica charantia was used as the raw material to prepare polypeptide.Appropriate conditions of separation and purification were determined by orthogonal test.The results show that the best conditions are as follows：dosage of enzyme 4%;enzymolysis temperature 45 ℃,and enzymolysis time 1 h,liquid to material ratio 2.Under above conditions,yield of polypeptide can be increased by 19.7%.Sephadex G-50 was used to purify polypeptide.The process of separation and purification is simply,feasible and low cost,and it is suitable for industrial production |
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| Keywords: | Momordica charantia Polypeptide Separation Cellulase |
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