乳酸菌源纳米硒对小鼠抗氧化性能的影响

硒是人体必需的重要微量元素之一,在生物活性和毒性之间范围极端狭窄,容易接近或进入了硒的毒性范围,但纳米硒具有较强的高效高安全优势。本研究利用对亚硒酸钠有较强耐受性的一株编号LA4乳酸菌株进行亚硒酸钠还原反应,制备纳米硒。通过对昆明系小鼠灌胃不同浓度纳米硒进行实验:对照组、低剂量组、中剂量组和高剂量组,研究分析乳酸菌LA4源纳米硒对小鼠抗氧化性能的影响。经SEM-EDX测试得到乳酸菌LA4能将亚硒酸钠还原成纳米尺寸的球状颗粒,粒径基本≤200nm。乳酸菌LA4源纳米硒对小鼠全血、睾丸中的GSH-PX的活性具有极显著提高作用（p<0.01）,对小鼠大脑、肝脏以及脾脏等重要器官组织T-AOC和POD活力也具有极显著提高作用（p<0.01）;乳酸菌LA4源纳米硒极显著的提高小鼠血清中T-SOD活力（p<0.01）,有利于降低小鼠血清及重要器官组织的MDA含量;因此乳酸菌LA4源纳米硒对小鼠抗氧化性能具有提高作用,并且乳酸菌LA4源纳米硒添加水平就算增加到1.0mg/kg·bw时,仍对小鼠抗氧化性能具有提高作用。 

Effect of lactic acid bacterially synthesized Se (0) nanoparticles on oxidation resistance in mice

Selenium ( Se) is one of the important and essential trace elements in human body, but the range between biological activity and toxicity of Se is extremely narrow, so it's easy to show its toxicity.However, nano- Se has an advantage of good security and efficient.In recent study, sodium selenite was reduced to nano- Se by LA4 strain which had high tolerance with sodium selenite. The nano- Se were given to Kunming mice by gavage 0, 0.2 ( low dose group) , 0.5 ( middle dose group) and 1.0mg / kg·bw ( high dose group) nano- Se respectively. The effect of LA4 lactic acid bacterially synthesized nano- Se on oxidation resistance of mice was researched. The result of Scanning electron microscopy- energy dispersive X- ray spectroscopy ( SEM- EDX) showed that sodium selenite was reduced into spherical particles in nanoscale ( about less than200nm) by LA4 lactic acid bacteria. LA4 lactic acid bacterially synthesized nano- Se could improve the activity of glutathione peroxidase ( GSH- PX) of whole blood and testis in mice ( p < 0.01) obviously, and also improve total antioxidative capacity ( T- AOC) and peroxidase ( POD) of important organs, such as brain, liver, spleen and so on, in mice ( p < 0.01) obviously.Moreover, LA4 lactic acid bacterially synthesized nano- Se could enhance the activity of total superoxide dismutase ( T- SOD) of serum in mice ( p < 0.01) , it also could reduce the content of malondialdehyde ( MDA) of serum and important organs in mice.In a conclusion, LA4 lactic acid bacterially synthesized nano- Se couldimprove the oxidation resistance of mice, and the oxidation resistance of mice enhanced with the content of LA4 lactic acid bacterially synthesized nano- Se increasing. But when the content of LA4 lactic acid bacterially synthesized nano- Se increased to1.0mg / kg·bw, also have oxidation resistance