| α-乙酰乳酸脱羧酶稳定剂的筛选 |
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| 引用本文： | 阚振荣,田新生,赵春海. α-乙酰乳酸脱羧酶稳定剂的筛选[J]. 食品工业科技, 2005, 26(9): 78-79 |
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| 作者姓名： | 阚振荣 田新生 赵春海 |
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| 作者单位： | 河北大学生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省重点攻关项目(94245509D)。 |
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| 摘 要： | 选用大分子有机物、醇类、无机盐等十余种物质为备选稳定剂,分别进行单因子的不同浓度实验,考察不同物质对乙酰乳酸脱羧酶热稳定性的影响。在此基础上筛选具备高效保护作用的因子,再通过正交实验设计复合因子实验,选取最佳组合,然后再利用最佳组合在不同溶剂系统中进行了酶的储存实验,在储存实验的四种溶剂系统中,其中一种不论在常温下或者冰箱条件下都显示出很好的保护作用。
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| 关 键 词： | α-乙酰乳酸脱羧酶 稳定剂 筛选 热稳定性 |
| 文章编号： | 1002-0306(2005)09-0078-03 |
| 修稿时间： | 2004-12-14 |
Stabilizer screening for α-Acetolactate decarboxylase |
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| Kan Zhenrong et al. Stabilizer screening for α-Acetolactate decarboxylase[J]. Science and Technology of Food Industry, 2005, 26(9): 78-79 |
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| Authors: | Kan Zhenrong et al |
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| Affiliation: | Kan Zhenrong et al |
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| Abstract: | Organic compounds, alcohols and inorganic salts etc. were selected as stabilizers used at various concentration in single factor experiments. By studying effects of different substances on a -ALDC the most effective protecting substances were screened out. The best combination of these substances was identified by orthogonal experiment. Stability tests of the enzyme in four solvent systems with the presence of the best combination were done and the results show among the four solvent systems, one showed good protection both at room temperature and refrigerated condition. |
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