| 缓释微球乙型肝炎疫苗的初步研究 |
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| 引用本文： | 吴晓蓉,贾文祥,邝玉,张再蓉,任娅,刘莉,朱雯,李孝红,邓先模.缓释微球乙型肝炎疫苗的初步研究[J].粉末涂料与涂装,2000,13(2):89-91. |
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| 作者姓名： | 吴晓蓉 贾文祥 邝玉 张再蓉 任娅 刘莉 朱雯 李孝红 邓先模 |
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| 作者单位： | [1]华西医科大学基础医学院微生物学教研室 [2]中国科学院成都有机化学所高分子材料室 |
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| 基金项目： | 本课题受国家自然科学基金(资助编号：29774034)和国家卫生部科研基金(资助编号：96-1-244)资助 |
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| 摘 要： | 目的 研制缓释微球乙型肝炎疫苗。方法 用聚－ＤＬ－乳酸－聚乙二醇共聚物（ＰＥＬＡ）为材料，包裹乙 型肝炎表面抗原（ＨＢｓＡｇ），制成缓释微球疫苗。微球的粒径均小于５ｕｍ，平均粒径为２．１７ｕｍ，抗原包裹量为１．２５％， 包裹率为６０－８０％。用 ＳＤＳ－ＰＡＧＥ检测抗原，以微球疫苗免疫 ＢＡＬＢ／ｃ。小鼠。结果 ＨＢｓＡｇ的结构包裹前后是一致 的，小鼠经皮下注射单剂微球疫苗后，在第 １４ｗ，小鼠血清 ＩｇＧ滴度可达到铝佐剂疫苗相当的水平，且维持较高的滴 度。结论采用生物可降解的缓释微球作为乙肝疫苗载体系统具有潜在的优势。
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| 关 键 词： | 乙肝疫苗 缓释微球 免疫应答 |
| 修稿时间： | 1999-08-04 |
Preliminary Study on Controlled Release Microencapsulated Hepatitis B Vaccine |
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| Wu Xiaorong
,Jia Wenxiang
,Kuang Yu.Preliminary Study on Controlled Release Microencapsulated Hepatitis B Vaccine[J].Chinese Journal of Biologicals,2000,13(2):89-91. |
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| Authors: | Wu Xiaorong Jia Wenxiang Kuang Yu |
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| Affiliation: | Wu Xiaorong
,Jia Wenxiang
,Kuang Yu
(Depanmeat of Microbiology, West China University of Medical Sciences, Chengdu 610041) |
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| Abstract: | Objective To prepare controlled release microencapsulated hepatitis B vaccine.Methods HBsAg was encapsulated in the Micro-prepared from polyethylene glycol-poly- DL-lactide- (PELA ). The average diameter of the micro-particles was 2. 17um, and all of them were less than 5um. The encapsulation rate of HBsAg was 60%-80%. The HBsAg was detected by SDS-PAGE, and the encapsulated vaccine was used for immunizing BALB/c mice.Results No change of structure of HBsAg was observed before and after encapsulation,and,at the 14th week after being injected subcutaneously with a single dose of the encapsulated vaccine,the serum IgG titers of the mice reached a level equivalent to that of vaccine using aluminium adjuvant.Conclusion The biodegradable particles can be used as a candidate vector system of hepatitis B vaccine. |
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| Keywords: | Hepatitis B vaccine Controlled release micro-particle Immune response |
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