| 红霉素产生菌的诱变及培养基优化研究 |
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| 引用本文： | 左勇,李杨,任永利,鞠帅,谢晖,祁峰.红霉素产生菌的诱变及培养基优化研究[J].四川轻化工学院学报,2012(1):14-18. |
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| 作者姓名： | 左勇 李杨 任永利 鞠帅 谢晖 祁峰 |
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| 作者单位： | 四川理工学院生物工程学院,四川自贡643000 |
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| 摘 要： | 以红色糖多孢菌08L作为出发菌株进行诱变，选育高产菌株。结果表明，诱变得到的UL5菌株发酵水平比出发菌株提高12．6％。再通过培养基优化，其组成为淀粉3％，糊精3％，葡萄糖2％，黄豆饼粉3％，玉米浆1％，硫酸铵0．15％，碳酸钙0．6％，磷酸二氢钾0．02％的条件下，UL5菌株在诱变的基础上，发酵水平再提高15．2％。
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| 关 键 词： | 工色糖多孢茵 诱变 正交实验 发酵 |
Study on the Mutation of Erythromycin Productive Strain and Its Fermentation |
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| ZUO Yong,LI Yang,REN Yong-li,JU Shuai,XIE Hui,QI Feng.Study on the Mutation of Erythromycin Productive Strain and Its Fermentation[J].Journal of Sichuan Institute of Light Industry and Chemical Technology,2012(1):14-18. |
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| Authors: | ZUO Yong LI Yang REN Yong-li JU Shuai XIE Hui QI Feng |
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| Affiliation: | (School of Biotechnology Engineering, Sichuan University of Science ＆ Engineering, Zigong 643000, China) |
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| Abstract: | The Saccharopolyspora erythraea 08L is used as the starting strain. Processed by ultraviolet mutagenesis, the fermentation product of the mutated strains was increased by 12.6%. In addition, under the optimized culture medium which consisted of starch 3%, dextrin 3%, glucose 2% , soybean meal 3%, corn steep liquor 1%, （ NH4 ）2SO4 0.15%, CaCOz0. 6%, KH2PO40.02%, the fementation yield of UL5 strains was increased by 15.2%. |
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| Keywords: | Saccharopolyspora erythraea mutagenesis orthogonal test fermentation |
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