| 西地那非多克隆抗体的制备及特异性分析 |
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| 引用本文： | 熊 波,吴胜泽,苏焕斌,张 燕,刘 辉.西地那非多克隆抗体的制备及特异性分析[J].食品安全质量检测技术,2015,6(3):1022-1027. |
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| 作者姓名： | 熊 波 吴胜泽 苏焕斌 张 燕 刘 辉 |
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| 作者单位： | 广东产品质量监督检验研究院,广东产品质量监督检验研究院,广东产品质量监督检验研究院,广东产品质量监督检验研究院,广东产品质量监督检验研究院 |
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| 基金项目： | 广东省科技计划项目(2013B090600059)*通讯作者：刘辉,博士研究生,食品质量工程师,主要研究方向为食品安全与营养。E-mail：lh403@163.com 熊波，吴胜泽，苏焕斌，张燕，刘辉* |
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| 摘 要： | 目的为了研究开发一种具有高灵敏度、高特异性的西地那非(sildenafil,SD)多克隆抗体。方法本文通过人工合成西地那非半抗原(SDH)和西地那非抗原(SDH-BSA),然后经免疫兔子,制备了SD多克隆抗体,并对制得的抗体的特异性进行了分析。结果免疫后产生了SD多克隆抗体,抗体的效价为3.8×105,线性检测范围为0.02~3.8 ng/m L,抗体除与瓦地那非具有较高的交叉反应率外,与红地那非、他地那非等结构类似物交叉反应率均较低。结论采用本方法制备的SD多克隆抗体具有较高的特异性和灵敏度,可用于SD快速检测试剂盒的开发。
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| 关 键 词： | 西地那非 多克隆抗体 酶联免疫 |
| 收稿时间： | 2015/1/19 0:00:00 |
| 修稿时间： | 2015/3/17 0:00:00 |
Preparation and specificity analysis of sildenafil polyclonal antibody |
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| XIONG BO,WU Sheng-Ze,SU Huan-Bin,ZHANG Yan and LIU Hui.Preparation and specificity analysis of sildenafil polyclonal antibody[J].Food Safety and Quality Detection Technology,2015,6(3):1022-1027. |
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| Authors: | XIONG BO WU Sheng-Ze SU Huan-Bin ZHANG Yan and LIU Hui |
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| Affiliation: | Guangdong Provincial Product Quality Supervision and Inspection Institute,Guangdong Provincial Product Quality Supervision and Inspection Institute,Guangdong Provincial Product Quality Supervision and Inspection Institute,Guangdong Provincial Product Quality Supervision and Inspection Institute,Guangdong Provincial Product Quality Supervision and Inspection Institute, Shunde |
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| Abstract: | Objective To prepare high sensitivity and specificity polyclonal antibody against sildenafil (SD). Methods Sildenafil hapten (SDH) and Sildenafil antigen (SDH - BSA) were be prepared by artificial. Then, through immuned rabbits the SD polyclonal antibody were be prepared. Result After immunity, the polyclonal antibody against SD was generated. The titer of the antibody was 3.8×105. The linear detection of the polyclonal antibody were range from 0.02 ng/mL to 3.8 ng/mL. The polyclonal antibody has high cross reaction rate to vardenafil, but has low response rate to hongdenafil and Calais. Conclusion Using this method prepared SD polyclonal antibody has high specificity and sensitivity, and it can be applied to the development of SD rapid detection kit. |
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| Keywords: | Sildenafil Polyclonal antibody ELISA |
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