| 途径工程生产1,3-丙二醇的研究进展 |
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| 引用本文： | 杨登峰,韦宇拓,杜丽琴,黄日波. 途径工程生产1,3-丙二醇的研究进展[J]. 现代化工, 2004, 24(11): 24-26 |
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| 作者姓名： | 杨登峰 韦宇拓 杜丽琴 黄日波 |
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| 作者单位： | 广西大学生物技术实验中心,广西,南宁,530005 |
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| 基金项目： | 国家高技术研究发展计划("863"计划)资助项目(2003AA001039) |
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| 摘 要： | 利用基因工程技术生产1,3-丙二醇,特别是途径工程的利用已取得了重大突破。利用生物技术生产1,3-丙二醇的成本比化学法的低25％,它将逐步实现工业化生产。介绍了一步法生产1,3-丙二醇的超级基因工程菌所需的3-磷酸甘油脱氢酶(GPD1)、甘油3-磷酸酶(GPP2)、甘油脱水酶(dhaB)和1,3~丙二醇氧化还原酶(dhaT),并对关键酶dhaB进行了分析,同时介绍了穿梭质粒和甘油-3-磷酸酶积累对生产的影响。最后,对今后的研究重点和策略进行了探讨。
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| 关 键 词： | 1 3-丙二醇 基因工程菌 途径工程 甘油脱水酶 |
| 文章编号： | 0253-4320(2004)11-0024-03 |
| 修稿时间： | 2004-05-20 |
Advances in production of 1,3-propanediol by pathway engineering |
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| YANG Deng-feng,WEI Yu-tuo,DU Li-qin,HUANG Ri-bo. Advances in production of 1,3-propanediol by pathway engineering[J]. Modern Chemical Industry, 2004, 24(11): 24-26 |
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| Authors: | YANG Deng-feng WEI Yu-tuo DU Li-qin HUANG Ri-bo |
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| Abstract: | The technology for production of 1,3-propanediol by the gene engineering has been broken through, especially by the pathway engineering. The cost of production was 25 % lower than that by the traditional chemical production. The industrialized production will be realized in the future. Glycerol 3-phosphate dehydrogenase(GPD1) , glycerol 3-phosphatase (GPP2), glycerol dehydratase (dhaB) and 1,3-propanediol oxidroreductase (dhaT) are introduced,which construct the super gene engineering for producing the 1,3-propanediol.The key enzyme,dhaB,are analyzed.The effect of the accumulation of shuttle plas-mid and glycerol 3-phosphatse on production is introduced.Finally,the focus and the strategy are also discussed for the future. |
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