化妆品中维生素D2、维生素D3含量的测量不确定度评定

对《化妆品安全技术规范》(2015年版)中有关维生素D2和维生素D3高效液相色谱法含量测定进行了不确定度评定,建立了数学模型,考察了不确定度的来源并给出了量化结果,计算出合成标准不确定度和扩展不确定度。结果表明,当置信区间为95%,包含因子k=2,被测化妆品中维生素D2和维生素D3含量分别为48.84μg/g和45.76μg/g时,其相应标准扩展不确定度分别为10.36μg/g和9.71μg/g。最后讨论了测量不确定度的主要来源,并提出了改进建议。

Evaluation of Measurement Uncertainty in the Determination of Vitamin D2 and Vitamin D3 in Cosmetics

Uncertainties from the determination procedure of vitamin D2 and vitamin D3 in "Safety and Technical Standards for Cosmetics"(2015 Edition) by high performance liquid chromatography (HPLC) were assessed. The mathematical model was established, and each source and uncertainty component were analyzed. The combined standard uncertainty and expanded uncertainty were reported at last. The results showed that the expanded uncertainties were 10.36 μg/g and 9.71 μg/g (95% confidence level, k=2) when the content of Vitamin D2 and Vitamin D3 in cosmetics were 48.84 μg/g and 45.76 μg/g. The main sources for the uncertainty were discussed, and suggestions for improvement were also introduced.