L-[S-~(11)C-甲基]-蛋氨酸的合成及HPLC分析

采用还原碘化法合成11 CH3I,固相法合成11 C-MET,HPLC分析产品放化纯度,并合成11 CH3OH和11CH3I,利用HPLC分析验证11 C-MET中杂质的成分,探讨溶解前体的NaOH浓度和有机溶剂用量对11 C-MET质量的影响。结果表明,先用0.1mL 2mol/L NaOH溶解前体,再与0.1mL无水乙醇混匀后装入C18柱,可以提高11 C-MET的产率及放化纯度。

Preparation and HPLC Analysis of L-[S-methyl-11 C]Methionine

11C‐MET was synthesized by the solid phase reaction of 11CH3I and precursor L‐homocysteine ,and the radiochemical purity of 11C‐MET was analyzed by HPLC ,11C‐methanol and 11C‐methyl iodide were prepared and analyzed ,respectively .The primary impurity of the product were analyzed . The influence on the quality of product was investigated by changing the concentration of sodium hydroxide solution and the solvent of dissolving precursor .The results showed that 11C‐MET with higher yield and good radiochemical purity would obtain by using 0.1 mL 2 mol/L NaOH to dissolve the pre‐cursor ,then injecting it into the C18 column after blending 0.1 mL ethanol .