| 谷氨酸棒杆菌argB基因的克隆及其在大肠杆菌中表达的初步研究 |
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| 引用本文： | 陈雪岚,许正宏,陶文沂.谷氨酸棒杆菌argB基因的克隆及其在大肠杆菌中表达的初步研究[J].食品科学,2005,26(1):31-35. |
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| 作者姓名： | 陈雪岚 许正宏 陶文沂 |
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| 作者单位： | 江南大学教育部工业生物技术重点实验室,江苏,无锡,214036 |
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| 基金项目： | 国家“十五”攻关项目(BE2001041) |
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| 摘 要： | 根据GenBank报道的谷氨酸棒杆菌ATCC 13032序列，设计并合成一对引物，利用温度梯度PCR仪，获得了argB基因全长。将其克隆到pGEM-T-Easy载体中，经测序鉴定后定向克隆至表达载体pGEX-4T-1中，构建了pGEX-argB表达质粒。
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| 关 键 词： | 谷氨酸棒杆菌 argB基因 克隆 表达 |
| 文章编号： | 1002-6630(2004)12-0031-05 |
Analysis of the argB Sequence from Corynebacterium Glutamicum and the Pilot Study on the Expression of the Gene in Escherichia coli |
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| CHEN Xue-lan,XU Zheng-hong,TAO Wen-yi.Analysis of the argB Sequence from Corynebacterium Glutamicum and the Pilot Study on the Expression of the Gene in Escherichia coli[J].Food Science,2005,26(1):31-35. |
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| Authors: | CHEN Xue-lan XU Zheng-hong TAO Wen-yi |
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| Abstract: | The argB sequence was obtained by designing and synthesizing the pair of primer, according to the report of GenBank about the sequence of Corynebacterium glutamicum, assayed by the temperature grads PCR instrument. The argB gene was cloned into plasmid pGEM-T-Easy. The recombinant strain harboring the pGEM-argB plasmid was verified by PCR reaction, plasmid extraction with alkaline method, and digestion with restriction endonuclease BamHI and EcoRI and DNA sequencing. An expressing plasmid pGEX-argB was constructed by the directional cloning of the targeted DNA into the plasmid pGEX-4T-1. |
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| Keywords: | Corynebacterium glutamcium argB gene clone expression |
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