纳米CdS∶Cu双酶膜葡萄糖生物传感器

将纳米CdS∶Cu颗粒加入到葡萄糖酶(GOD)和辣根过氧化物酶(HRP)双酶膜中,与导电聚合物聚邻苯二胺(PoPD)经电化学聚合反应而固定此两酶,制备了电流型纳米CdS∶Cu颗粒双酶膜葡萄糖生物传感器,分析了CdS∶Cu纳米颗粒对传感器电流响应的影响,进行了传感器的性能测定。实验表明,引入CdS∶Cu纳米粒子和PoPD后可显著改进传感器响应性能,线性范围为0.55~9.2 mmol/L,检测下限为0.55 mmol/L,响应时间为20 s。稳定工作215天,传感器活性指标无显著变化,且抗干扰性强。

Study on CdS: Cu Nano-particles Dual-enzyme Membrane Glucose Biosensors

CdS : Cu nano-particles were added to the glucose enzyme (GOD) and horseradish peroxidase (HRP) dual-enzyme membrane, and then reacted with conducting polymer poly o-phenylenediamine (PoPD) via electrochemical polymerization to fix these two enzymes and to prepare the current nano-CdS : Cu particle dual-enzyme membrane glucose biosensors. The response to electric current of CdS : Cu nano-particles in the biosensors was analyzed, and the performance of the biosensors was tested. The experimental results showed that the introduction of nano-particles and PoPD significantly enhanced the sensor response sensitivity. The linear range expanded to 0. 55 ~ 9. 2 mmol/L, and the detection limit reduced to 0. 55 mmol/L. The response time was 20 s. During 215 days, the biosensor worked steadily with no significant changes in activity and exhibited strong anti-interference property.