| 鱼降压肽的大孔吸附树脂分离及其活性稳定性 |
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| 引用本文： | 陈季旺,孙勤,夏文水.鱼降压肽的大孔吸附树脂分离及其活性稳定性[J].食品科学,2009,30(18):25-28. |
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| 作者姓名： | 陈季旺 孙勤 夏文水 |
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| 作者单位： | 1.武汉工业学院食品科学与工程学院，湖北武汉 430023；2.江南大学食品学院，江苏无锡 214122 |
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| 基金项目： | 国家“863”计划重大项目(2006AA09Z444;2007AA091802); 省部产学研结合项目(2007A090302059) |
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| 摘 要： | 采用大孔吸附树脂对碱性蛋白酶水解、水解度为34.52% 的鱼降压肽进行分离。结果表明，DA201-C 型大孔吸附树脂对鱼降压肽吸附性最好，乙醇体积分数为75% 时洗脱下来的组分具有最高的ACE 抑制活性，其IC50为1.52mg/ml。鱼降压肽中灰分由分离前的9.47% 减少到分离后的2.34%，肽和蛋白质含量分别由分离前的72.81%和81.26% 增加到分离后的80.24% 和92.42%。活性稳定性分析显示，胃蛋白酶和胰酶、中性和低酸性pH 值、55℃以下温度以及1000mmol/L 以内的NaCl 浓度对鱼降压肽的ACE 抑制活性影响较小。
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| 关 键 词： | 草鱼 降压肽 大孔吸附树脂 血管紧张素转化酶活性 |
| 收稿时间： | 2009-03-02 |
Macroporous Adsorption Resin Isolation and Stability of Angiotensin-converting Enzyme (ACE) Inhibitory Activity of Antihypertensive Peptides Derived from Grass Carp Protein |
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| CHEN Ji-wang,SUN Qing,XIA Wen-shui.Macroporous Adsorption Resin Isolation and Stability of Angiotensin-converting Enzyme (ACE) Inhibitory Activity of Antihypertensive Peptides Derived from Grass Carp Protein[J].Food Science,2009,30(18):25-28. |
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| Authors: | CHEN Ji-wang SUN Qing XIA Wen-shui |
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| Affiliation: | 1. School of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China ；
2. School of Food Science and Technology, Jiangnan Unviersity, Wuxi 214122, China |
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| Abstract: | Various macroporous adsorption resins were used for the isolation of antihypertensive peptides from grass carp protein hydrolysate with the hydrolysis degree of 34.52% and the stability of angiotensin-converting enzyme (ACE) inhibitory activity of the peptides was analyzed. Among 8 types of macroporous adsorption resin tested, DA201-C had the best adsorption capability. The peptide fraction eluted by 75% ethanol displayed the highest ACE inhibitory activity with the IC50 of 1.52 mg/ml. After isolation, the ... |
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| Keywords: | grass carp antihypertensive peptides macroporous adsorption resin angiotensin-converting enzyme (ACE) inhibitory activity |
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