Matrix-implanted laser desorption/ionization mass spectrometry |
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Authors: | Novikov Alexey Caroff Martine Della-Negra Serge Lebeyec Yvon Pautrat Michèle Schultz J Albert Tempez Agnès Wang Hay-Yan J Jackson Shelley N Woods Amina S |
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Affiliation: | Institut de Physique Nucléaire, CNRS-IN2P3, 91406 Orsay, France, Equipe Endotoxines, UMR 8619 du CNRS, IBBMC, Université de Paris-Sud, 91405 Orsay, France. |
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Abstract: | The implantation of low-velocity massive gold clusters is shown to be a method of choice for homogeneous incorporation of a metallic matrix into the near-surface region of a solid biopolymer for subsequent laser desorption/ionization (LDI) MS analysis. Matrix implanted (MI)LDI spectra from cluster-implanted pure test peptide or tissue exhibit molecular ion peaks similar to those observed by matrix-assisted LDI. Moreover, the ion emission is very reproducible from any spot on the surface of these test samples. MILDI promises to be a powerful technique for mass spectrometric analysis of native biological samples as demonstrated by the first results on rat brain tissues. |
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