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Immobilization of albumin on magnetite nanoparticles |
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| Authors: | Esra Maltas Mustafa Ozmen Hasibe Cingilli Vural Salih Yildiz Mustafa Ersoz |
| Affiliation: | 1. Istanbul Technical University, Faculty of Science and Letters, Department of Physics Engineering, 34469, Maslak, Istanbul, Turkey;2. National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan;1. AmirKabir University of Technology, Energy Engineering and Physics Faculty;2. Shahid Beheshti university of Tehran, Radiation Application Department;1. Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, 11000 Belgrade, Serbia;2. Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia;3. The “Vin?a” Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia;4. Institute of Chemistry – Analytical Chemistry, Karl-Franzens University Graz, Graz, Austria |
| Abstract: | The magnetite (Fe3O4) nanoparticles were prepared by the co-precipitation of ferrous and ferric salts with NH4OH, and then modified with 3-aminopropyltriethoxysilane (APTES) by silanization reaction and subsequent reaction with glutaraldehyde (GA) to obtain functional groups on their surface. The influence of different terminated groups on protein binding was studied with bare and modified magnetite nanoparticles. Amine terminated magnetite nanoparticles were shown the highest binding ability for immobilization process compared to Fe3O4 NPs and GA bonded NPs. This binding ability was shown by using sodium dodecyl polyacrylamide gel electrophoresis technique (SDS-PAGE). Albumin attached magnetite nanoparticles were also examined by Scanning Electron Microscopy (SEM). |
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