正交设计法优化Bt发酵培养基

采用正交实验方法,进行摇瓶发酵培养基筛选试验来优化苏云金芽孢杆菌发酵培养基,通过测量发酵液吸光度值来确定不同农副产品培养基成分对苏云金芽孢杆菌发酵的影响,从而使培养基优化过程简单易行。实验结果表明,不同培养基成分配比对发酵效果影响差别很大,试验所得的最佳培养基组合为:玉米粉2.0%、豆饼粉3.5%、鱼粉2.0%、花生饼粉2.0%、淀粉1.0%、K2HPO40.3%、CaCO30.3%。

The Method of Orthogonal Design Optimizes the Fermentation Culture Media of Bacillus thuringiensis

The optimization experiment of medium of shaking flasks fermentation of Bacillus thuringiensis was conducted by the orthogonal design. The effects of agricultural by-products on the fermentation were acquired by measuring the absorbency of fermentation liquids, which makes the optimization process easy and feasible. By orthogonal design experiment, the best medium formula was got, which was corn flour 2.0%, soy flour 3.5%, fish meal2.0%, peanut flour 2.0%, amylum 1.0%, K2HPO4 0.3%, CaCO3 0.3% .