苔干水溶性粗多糖提取工艺及理化性质的研究

本文采用热水浸提、醇沉法提取苔干多糖,对苔干多糖提取工艺和一般理化性质进行了研究。选择浸提固液比、温度和时间作为单因素进行梯度实验,确定其条件范围,再通过正交实验L9(34)进一步确定苔干水溶性粗多糖最佳提取工艺条件。结果表明最佳提取工艺为:固液比1:40、浸提温度90℃、浸提时间3h、反复浸提2次。理化性质结果表明提取的苔干多糖中含有多肽或氨基酸,为蛋白质复合多糖。

Study on the Extraction and Physical - chemical Properties on the Lactuca Sativa L.Var. Yemena X.S.Shen et X.P.Zhang Polysaccharides

This paper studied by using water extraction,alcohol deposit to extract the Lactuca sativa L.var yemena X.S.Shen et X.P.Zhang polysaccharides from Lactuca sativa L.var yemena X.S.Shen et X.P.Zhang,and studied the conditions of extraction and physical-chemical property.The effects of three factors such as the proportion of water volume,the temperature of extraction,the time of extraction on the extraction of Lactuca sativa L.var yemena X.S.Shen et X.P.Zhang.Polysaccharides were investigated.With the orthogonal design of L9(34),the optimum technological conditions of extraction of polysaccharides from Lactuca sativa L.var yemena X.S.Shen et X.P.Zhang were obtained as follows:adding 50 times water as much as Lactuca sativa L.var yemena X.S.Shen et X.P.Zhang in 90℃ for 3 hours by 2 times and material to liquid 1:40 extraction.The results of some physical and chemical properties showed that there were tintin or amino acid in Lactuca sativa L.var yemena X.S.Shen et X.P.Zhang polysaccharide.Therefore,this polysaccharide is protein compound polysaccharides.