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Four Weeks of Aerobic Training Affects Cardiac Tissue Matrix Metalloproteinase,Lactate Dehydrogenase and Malate Dehydrogenase Enzymes Activities,and Hepatorenal Biomarkers in Experimental Hyperhomocysteinemia in Rats
Authors:Dusan Todorovic  Marija Stojanovic  Ana Medic  Kristina Gopcevic  Slavica Mutavdzin  Sanja Stankovic  Dragan Djuric
Affiliation:1.Faculty of Medicine, Institute of Medical Physiology “Richard Burian”, University of Belgrade, 11000 Belgrade, Serbia; (D.T.); (M.S.); (S.M.);2.Faculty of Medicine, Institute of Chemistry in Medicine “Prof. Dr. Petar Matavulj”, University of Belgrade, 11000 Belgrade, Serbia; (A.M.); (K.G.);3.Centre of Medical Biochemistry, Clinical Centre of Serbia, 11000 Belgrade, Serbia;
Abstract:The aim of this study was to investigate the effect of the application of homocysteine as well as its effect under the condition of aerobic physical activity on the activities of matrix metalloproteinases (MMP), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cardiac tissue and on hepato-renal biochemical parameters in sera of rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C: 0.9% NaCl 0.2 mL/day subcutaneous injection (s.c.); H: homocysteine 0.45 µmol/g b.w./day s.c.; CPA saline (0.9% NaCl 0.2 mL/day s.c.) and a program of physical activity on a treadmill; and HPA homocysteine (0.45 µmol/g b.w./day s.c.) and a program of physical activity on a treadmill. Subcutaneous injection of substances was applied 2 times a day at intervals of 8 h during the first two weeks of experimental protocol. Hcy level in serum was significantly higher in the HPA group compared to the CPA group (p < 0.05). Levels of glucose, proteins, albumin, and hepatorenal biomarkers were higher in active groups compared with the sedentary group. It was demonstrated that the increased activities of LDH (mainly caused by higher activity of isoform LDH2) and mMDH were found under the condition of homocysteine-treated rats plus aerobic physical activity. Independent application of homocysteine did not lead to these changes. Physical activity leads to activation of MMP-2 isoform and to increased activity of MMP-9 isoform in both homocysteine-treated and control rats.
Keywords:homocysteine   exercise   lactate dehydrogenase   malate dehydrogenase   matrix metalloproteinase   hepato-renal markers   heart   rat
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