白腐菌预处理甘蔗渣促进水解的研究

利用一株产木质素降解酶的白腐菌Ceripriopsis sp.,以固态发酵形式对甘蔗渣进行预处理,选择性降解其中的木质素,从而提高其水解效率。甘蔗渣固态发酵过程中,发酵温度、培养基成分、含水量和初始pH值对酶活和糖收率都有影响。含水量是对糖收率影响最大的因素,其次是麸皮添加量,再次是温度,而初始pH值影响最小。甘蔗渣固态发酵的最佳条件为:培养基中含水量为80%,麸皮含量为5%,初始pH=4.0时28℃发酵14d,获得的糖产率最大,达20.6%。

Research on pretreatment by white-rot Fungi for the contribution to hydrolysis of bagasse

The research is focused on pretreatment on bagasse by solid-state fermentation with selective white-rot fungus Ceripriopsis sp.which degrades of lignin selectively,thereby enhancing the hydrolysis efficiency.The fermentation temperature,medium composition,moisture content and initial pH have an impact on enzyme activity and sugar yield in solid-state fermentation process.The moisture is the most influential factor on the sugar yield,followed by wheat bran added,temperature,and the initial pH with minimal impact.The best conditions for the solid-state fermentation of bagasse are medium moisture of 80%,5% of wheat bran,initial pH 4.0 at 28 ℃ for 14 days to obtain the sugar yield of 20.6%.