正交试验优选半枝莲总黄酮提取工艺研究

通过L16(45)正交试验优选超声辅助半仿生提取半枝莲总黄酮工艺条件。结果显示,芦丁标准品浓度在0.01~0.05 mg/mL范围内与吸光度呈良好线性关系(r=0.9993);最佳提取条件:超声功率80 W、时间35 min、料液质量比1∶35、温度60℃、乙醇体积分数80%,此条件下总黄酮平均提取率最高,为3.01%。经验证结果可靠,采用超声辅助半仿生提取方法简单、安全、快速。

Opimization of Extraction of Total Flavonoids from Soutellaria Barbata by Orthogonal Test

Using orthogonal design method and adopting L16 （45） table, the optimum extraction process of total flavonoids from Scutellaria flavonoid was carried out by using ultrasound - assisted barbata semi - bionic extraction. The results showed that the concentration of turin standard 0. 01 -0. 05 mg/mL range and the absorbance was a good linear relationship （ r = 0. 9993 ）. The optimal process conditions were determined that ultrasonic power was 80 W, ultrasonic time was 35 min, the material - liquid ratio was 1 ： 35, extraction temperature was 60 ℃, and volume fraction of ethanol was 80%. Under this condition, the average extraction rate of total flavone was the highest of 3.01%. With the optimum conditions, it was a proven and reliable results. The ultrasound - assisted semi - bionic extraction method was simple, safe and fast.