Volatile lactones from streptomycetes arise via the antimycin biosynthetic pathway |
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Authors: | Riclea Ramona Aigle Bertrand Leblond Pierre Schoenian Ilka Spiteller Dieter Dickschat Jeroen S |
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Affiliation: | 1. Institut für Organische Chemie, Technische Universit?t Braunschweig, Hagenring 30, 38106 Braunschweig (Germany);2. Faculté des Sciences et Technologiques, Université de Lorraine, Boulevard des Aiguilettes, B.?P. 239, 54506 Vandoeuvre‐lès‐Nancy (France);3. Chemical Ecology/Biological Chemistry, University of Konstanz, 78457 Konstanz (Germany);4. Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans‐Kn?ll Strasse 8, 07745 Jena (Germany) |
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Abstract: | The volatiles released by several streptomycetes were collected by using a closed-loop stripping apparatus (CLSA) and analysed by GC-MS. The obtained headspace extracts of various species contained blastmycinone, a known degradation product of the fungicidal antibiotic, antimycin A(3b), and several unknown derivatives. The suggested structures of these compounds, based on their mass spectra and GC retention indices, were confirmed by comparison to synthetic reference samples. Additional compounds found in the headspace extracts were butenolides formed from the blastmycinones by elimination of the carboxylic acid moiety. Analysis of a gene knockout mutant in the antimycin biosynthetic gene cluster demonstrated that all blastmycinones and butenolides are formed via the antimycin biosynthetic pathway. The structural variation of the blastmycinones identified here is much larger than within the known antimycins, thus suggesting that several antimycin derivatives remain to be discovered. |
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Keywords: | biosynthesis GC–MS lactones streptomycetes synthesis |
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