Effect of chronic glucagon administration on lipoprotein composition in normally fed,fasted and cholesterol-fed rats |
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Authors: | Catherine Guettet Najmuddin Rostaqui Denis Mathe Bernard Lecuyer Nicole Navarro Bernard Jacotot |
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Affiliation: | 1. Unité de Recherches sur les Dyslipidémies et l’Atherosclérose, INSERM U 32 H?pital Henri-Mondor, 94010, Créteil, France
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Abstract: | Male adult Wistar rats received daily (at 9 a.m. and 5 p.m.) 10 μg of zinc-protamine glucagon by subcutaneous injection for
8 days. Plasma cholesterol levels were decreased by 36% in fed rats, 33% in cholesterol-fed rats and by 55% in fasted rats.
Lipoproteins were separated into 22 fractions by ultracentrifugation using a density gradient. Glucagon administration decreased
the cholesterol content in all lipoproteins except low density lipoprotein (LDL1) (1.006–1.040) and very low density lipoprotein (VLDL) from cholesterol-fed rats. The main decrease (−57 to −81%) was observed
in 1.050–1.100 g/mL lipoproteins (LDL2 and HDL2), which contained a large amount of apo E, while HDL3 cholesterol was not affected. Triacylglycerol levels were decreased only in chylomicrons and VLDL (−70%) of fed and cholesterol-fed
rats, while plasma and lipoprotein triacylglycerol levels were not changed in fasted rats treated with glucagon. In normally
fed rats glucagon administration increased by 42% the fractional catabolic rate of 125I]HDL2 while the absolute catabolic rate appeared to be unchanged. Glucagon seems to be a potent hypolipidemic agent affecting mainly
the apo E-rich lipoproteins. Its chronic administration limits lipoprotein accumulation which occurs upon cholesterol feeding. |
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