| 沉默生存素基因增强人肝癌细胞SMMC-7721放射敏感性 |
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| 引用本文： | 杨巍,尹小祥,曹建平,刘芬菊.沉默生存素基因增强人肝癌细胞SMMC-7721放射敏感性[J].辐射研究与辐射工艺学报,2010,28(6):352-356. |
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| 作者姓名： | 杨巍 尹小祥 曹建平 刘芬菊 |
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| 作者单位： | 苏州大学放射医学与公共卫生学院放射生物学教研室,苏州,215123 |
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| 基金项目： | 国家自然科学基金青年项目(30600160)资助 |
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| 摘 要： | 为探讨沉默生存素(Survivin)基因对人肝癌细胞SMMC-7721放射敏感性的影响,利用靶向Survivin基因的RNA干扰(RNA interference,RNAi)质粒pGenesil-survivin,采用阳离子脂质体介导法转染SMMC-7721细胞后48 h,分别以RT-PCR和Western blot法检测Survivin基因mRNA和蛋白表达,以四甲基偶氮唑盐(MTT)法检测细胞增殖活性,流式细胞术检测细胞周期和细胞凋亡,克隆存活实验检测细胞放射敏感性的变化。结果表明,转染质粒pGenesil-survivin后48 h,SMMC-7721细胞Survivin基因mRNA和蛋白表达水平与对照和阴性干扰组相比明显降低(p0.05);转染后第1—4 d,细胞增殖活性与对照组相比明显降低(p0.05或p0.01);转染后48 h,细胞阻滞于G2/M期(p0.001),凋亡百分率明显高于对照组(p0.001);转染后SMMC-7721细胞D0值减小,放射敏感性增强,增敏比为1.24。结果提示,沉默生存素基因可增强人肝癌细胞SMMC-7721放射敏感性。
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| 关 键 词： | RNA干扰 生存素 肝细胞癌 放射敏感性 |
Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721 |
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| YANG Wei,YIN Xiaoxiang,CAO Jianping,LIU Fenju.Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721[J].Journal of Radiation Research and Radiation Processing,2010,28(6):352-356. |
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| Authors: | YANG Wei YIN Xiaoxiang CAO Jianping LIU Fenju |
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| Affiliation: | YANG Wei YIN Xiaoxiang CAO Jianping LIU Fenju(Department of Radiobiology,School of Radiological Medicine and Public Health,Soochow University,Suzhou 215123,China) |
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| Abstract: | In order to investigate the effect of RNA interference silencing survivin gene on radio-sensitivity of human hepatoma SMMC-7721 cells,mRNA and protein expression of survivin gene in SMMC-7721 cells after transfection with pGenesil-survivin-HIF mediated by liposome were detected by RT-PCR and western blot respectively.Cell viability was detected by MTT assay.Cell cycle distribution and apoptosis of SMMC-7721 cells were detected by FCM assay.Radio-sensitivity of SMMC-7721 cells was determined by clonogenic as... |
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| Keywords: | RNA interference Survivin Hepatoma Radio-sensitivity |
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