| Thermotoga martima嗜热木聚糖酶化学修饰结果与其结构特性关系研究 |
| |
| 引用本文： | 苏樨州,蔡萍,刘红梅,严明.Thermotoga martima嗜热木聚糖酶化学修饰结果与其结构特性关系研究[J].计算机与应用化学,2009,26(10). |
| |
| 作者姓名： | 苏樨州 蔡萍 刘红梅 严明 |
| |
| 作者单位： | 南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,江苏,南京,210009 |
| |
| 基金项目： | 国家自然科学基金-广东联合基金项目(U0733001);;南京工业大学博士论文创新研究基金(BSCX200810,BSCX200809) |
| |
| 摘 要： | 应用化学修饰的实验方法,结合蛋白质结构信息的计算来研究酶蛋白中氨基酸残基化学修饰与结构信息之间的关系。以Thermotoga maritima嗜热木聚糖酶为对象,采用PDB数据库中的1VBR为模板计算其序列中色氨酸、谷氨酸、天冬氨酸的溶剂可及性、氢键、盐桥数等结构特性,并与该酶化学修饰的实验结果相对比。结果表明酶活性中心3个色氨酸中,可及性大的Trp802与Trp602两个残基对酶的活性影响较大;序列中谷氨酸与天冬氨酸的氢键、盐桥数较多,修饰其对酶的热稳定性有很大影响。此结果有助于深入了解蛋白质中与化学修饰有关的结构特性,并为基于蛋白质结构的酶蛋白改性奠定了基础。
|
| 关 键 词： | 嗜热木聚糖酶 化学修饰 结构特性 |
Study on the relationship between structural characteristics and chemical modification to Thermo-stable Xylanase from Thermotoga maritima |
| |
| Su Xizhou,Cai Ping,Liu Hongmei,Yan Ming.Study on the relationship between structural characteristics and chemical modification to Thermo-stable Xylanase from Thermotoga maritima[J].Computers and Applied Chemistry,2009,26(10). |
| |
| Authors: | Su Xizhou Cai Ping Liu Hongmei Yan Ming |
| |
| Affiliation: | State Key Laboratory of Materials-Oriented Chemical Engineering;College of Life Science and Pharmaceutical Engineering;Nanjing University of Technology;Nanjing;210009;Jiangsu;China |
| |
| Abstract: | Chemical modification and protein structure calculation methods were used to investigate the relationship between the chemical modification of amino-acid residues and their structure informations in protein.Choose thermo-stable xylanase from Thermotoga maritima as research object and 1VBR from PDB as template,computing the structural characteristics of 1VBR such as accessibility,hydrogen bonding network,and salt bridges.Then compare these structural characteristics with experimental data of chemical modific... |
| |
| Keywords: | thermo-stable xylanase chemical modification structural characteristics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
