| Biolog鉴定产氢发酵细菌及其产氢能力的研究 |
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| 引用本文： | 吴薛明. Biolog鉴定产氢发酵细菌及其产氢能力的研究[J]. 应用化工, 2006, 35(7): 491-493 |
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| 作者姓名： | 吴薛明 |
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| 作者单位： | 南京中医药大学,药学院,江苏,南京,210046;南京工业大学,制药与生命科学学院,江苏,南京,210009 |
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| 摘 要： | 采用厌氧培养技术,从厌氧活性污泥中分离得到一株产氢发酵细菌。利用B iolog自动菌种鉴定仪对该产氢发酵细菌作了鉴定分析,确定了其在细菌学上的分类地位,新分离菌株C lostridium papyrosolvens为生物制氢分离鉴定纯产氢菌种提供了指导,该菌株为专性厌氧杆菌,蔗糖发酵液体末端主要产物为乙醇、乙酸,气相产物为H2和CO2,代谢特征为乙醇型发酵,在pH 6.0和36℃条件下最大产氢量为72 mL H2/g蔗糖。
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| 关 键 词： | 产氢菌 活性污泥 分离鉴定 Biolog |
| 文章编号： | 1671-3206(2006)07-0491-03 |
| 收稿时间： | 2006-05-17 |
| 修稿时间： | 2006-05-17 |
Study on determination of hydrogen production bacteria by Biolog and its ability of hydrogen production |
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| WU Xue-ming. Study on determination of hydrogen production bacteria by Biolog and its ability of hydrogen production[J]. Applied chemical industry, 2006, 35(7): 491-493 |
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| Authors: | WU Xue-ming |
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| Affiliation: | 1. Pharmaceutical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China; 2. College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China |
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| Abstract: | Anaerobic culture technique was adopted in this study to isolate a hydrogen producing bacteria in anaerobic activated sludge.This bacteria's classifying position in bacteriology was determined by Biolog,the strain of Clostridium papyrosolvens will be propitious to isolation and characterization pure bacteria in bio-hydrogen production.Clostridium papyrosolvens is obligate anaerobe bacillus,its main liquid fermentative products are ethanol and acetic acid;its gas fermentative production are H_2 and CO_2.Clostridium papyrosolvens's maximum capability of hydrogen production is 72 mL H_2/g sucrose at 36℃(pH(=6.0).) |
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| Keywords: | Biolog |
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