Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene |
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Authors: | MM Vickerman DG Heath DB Clewell |
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Affiliation: | Department of Cariology, School of Dentistry, University of Michigan, Ann Arbor 48109. |
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Abstract: | Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated. |
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