| 分光光度法测定板栗刺壳中的单宁含量 |
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| 引用本文： | 高龙,王全杰,许长通,张姝,范怀林. 分光光度法测定板栗刺壳中的单宁含量[J]. 西部皮革, 2011, 33(6): 16-19 |
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| 作者姓名： | 高龙 王全杰 许长通 张姝 范怀林 |
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| 作者单位： | 1. 烟台大学化学生物理工学院,山东,烟台,264005
2. 烟台大学化学生物理工学院,山东,烟台,264005;国家制革技术研究推广中心,山东,烟台,264005 |
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| 基金项目： | 烟台大学大学生科技创新基金资助项目 |
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| 摘 要： | 采用显色分光光度法测定板栗刺壳中单宁的含量的方法。在弱碱性条件下,板栗刺壳中单宁与钨酸钠-磷钼酸形成稳定的蓝色配合物,在766nm处测定显色液的吸光度确定单宁的含量。以单宁酸为标样,单宁量在0.0004～0.002mg/mL范围内服从比耳定律,回归曲线方程Y=47.913X-0.0503,相关系数R2=0.997。
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| 关 键 词： | 板栗刺壳 单宁 钨酸钠-磷钼酸 分光光度法 |
Quantitative Determination of Tannin in Chestnut Shell Thorns by Spectrophotometry |
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| GAO Long,WANG Quan-Jie,XU Chong-tong,ZHANG Shu,FAN Huai-lin. Quantitative Determination of Tannin in Chestnut Shell Thorns by Spectrophotometry[J]. Westl Eatbar, 2011, 33(6): 16-19 |
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| Authors: | GAO Long WANG Quan-Jie XU Chong-tong ZHANG Shu FAN Huai-lin |
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| Affiliation: | GAO Long1,WANG Quan-Jie1,2,XU Chong-tong1,ZHANG Shu1,FAN Huai-lin1(1.Chemistry and Biology College,Yaitai University,Yantai 264005,China,2.State Research and Promotion Center of Leather-making Technology,China) |
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| Abstract: | Spectrophotometry determination of tannin content in chestnut shell thorns was established with sodium tungstate-phosphomolybdate acid.Under weak alkaline condition,tannin and sodium tungstate-phosphomolybdate acid can form a stable blue complex,whose absorbance can be measured at 766 nm to determine the tannin concentration.With tannic acid as the standard sample,Beer's Law is obeyed in the concentration range of 0.0004-0.002 mg/mL of tannin,the regression equation was Y=47.913X-0.0503 and the correlation ... |
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| Keywords: | chestnut shell thorns tannin sodium tungstate-phosphomolybdic acid spectrophotometry |
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