| 氮氧自由基定点修饰牛血红蛋白的制备和抗自氧化研究 |
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| 引用本文： | 齐东来,王丹,李倩,王翔.氮氧自由基定点修饰牛血红蛋白的制备和抗自氧化研究[J].精细化工,2017,34(8). |
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| 作者姓名： | 齐东来 王丹 李倩 王翔 |
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| 作者单位： | 天津工业大学环境与化学工程学院,天津工业大学环境与化学工程学院,天津工业大学环境与化学工程学院,天津工业大学环境与化学工程学院 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘 要： | 摘要:以4-（N-马来酰亚胺基）-2,2,6,6-四甲基哌啶-1-氮氧自由基( Mal-Tempol) 与牛血红蛋白（Hb）为原料，制备了氮氧自由基定点修饰在巯基上的血红蛋白( Mal-Tempol-Hb)。利用分光光度法测定了氮氧自由基的修饰度，通过紫外光谱研究了有无叠氮化钠（NaN3）介导下，Hb和Mal-Tempol-Hb中高铁血红蛋白(MetHb)含量随时间变化的过程。结果表明，无介导下，3h时Mal-Tempol-Hb中MetHb的摩尔百分含量由修饰前的44%下降为修饰后的28%；在NaN3介导下，3h时Mal-Tempol-Hb中MetHb的摩尔百分含量从修饰前的50%下降为修饰后的32%。氮氧自由基的修饰有效地降低了血红蛋白的自氧化速率。
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| 关 键 词： | 血红蛋白 氮氧自由基 巯基 自氧化 高铁血红蛋白 |
| 收稿时间： | 2016/12/14 0:00:00 |
| 修稿时间： | 2017/4/4 0:00:00 |
Preparation of the Hemoglobin Site-specifically Modified with TEMPO and Study of its Antioxidation |
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| Qi Dong-lai,WANG Dan,LI Qian and WANG Xiang.Preparation of the Hemoglobin Site-specifically Modified with TEMPO and Study of its Antioxidation[J].Fine Chemicals,2017,34(8). |
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| Authors: | Qi Dong-lai WANG Dan LI Qian and WANG Xiang |
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| Affiliation: | Tianjin Polytechnic University,Tianjin Polytechnic University School of Environmental and Engineering,Tianjin Polytechnic University School of Environmental and Engineering,Tianjin Polytechnic University |
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| Abstract: | 4- (N-maleimide)-2,2,6,6-tetramethylpiperidine-1-oxyl (Mal-Tempol) and bovine hemoglobin (Hb) were used as raw materials and the Hemoglobin site-specifically modified on thiol group with nitrogen oxygen free radicals was synthesized. The modification degree of TEMPO was determined by spectrophotometric method. Moreover, the autoxidation of Hb and Mal-Tempol-Hb was discussed with or without the presence of sodium azide (NaN3).The experimental results demonstrated that the autoxidation rate of Mal-Tempol-Hb was much lower than Hb either with or without sodium azide, and the modification effectively improved the antioxidant capacity of Hb. |
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| Keywords: | hemoglobin TEMPO thiol group autoxidation MetHb |
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